Mouse Protein Tyrosine phosphatase Receptor F (PTPRF) Monoclonal Antibody | anti-PTPRF antibody

Protein Tyrosine Phosphatase Receptor F (PTPRF) Monoclonal Antibody

Cat. Num. AAA191363

• Gene Names: PTPRF; LAR; BNAH2
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Protein Tyrosine phosphatase Receptor F (PTPRF); Monoclonal Antibody; Protein Tyrosine Phosphatase Receptor F (PTPRF) Monoclonal Antibody; anti-PTPRF antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: This antibody recognizes human, mouse, and rat PTPRF.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 1907

• Applicable Applications for anti-PTPRF antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of ~85kDa is detected which represents the subunit containing transmembrane and intracellular domains.; IF: 10ug/ml; Dilute in PBS or medium that is identical to that used in the assay system.
• Immunogen: Fusion protein corresponding to aa 1315-1607 (cytoplasmic C-terminus) of human PTPRF. This sequence is 97% identical in rat and mouse, 80% identical in PTPRD and PTPRS, and >50% identical in PTPRM and PTPRK.
• Preparation and Storage: This product is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of PTPRF in rat brain lysate at 4ug/ml. )

Testing Data

(Detection of PTPRF in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) PTPRF stain. These are recommended concentrations. Endusers should determine optimal concentrations for their application.)

Related Product Information for anti-PTPRF antibody

PTPRF, like other members of the protein tyrosine phosphatase (PTP) family, is a signaling molecule that regulates a variety of cellular processes. PTPRF possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and represents a receptor-type PTP. The extracellular region contains three Ig-like domains and nine non-Ig like domains similar to that of neural-cell adhesion molecule. PTPRF functions in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of betacatenin signaling. An increased expression level of PTPRF has been found in the insulinresponsive tissue of obese, insulin-resistant individuals and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 109633041
• NCBI GeneID: 5792
• NCBI Accession #: NP_002831.2
• NCBI GenBank Nucleotide #: NM_002840.4
• UniProt Accession #: P10586; Q5T021; Q5T022; Q5W9G2; Q86WS0; D3DPX6; D3DPX7
• Molecular Weight: 211,687 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase F isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type F
• NCBI Official Symbol: PTPRF
• NCBI Official Synonym Symbols: LAR; BNAH2
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase F
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase F
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRF
• UniProt Synonym Gene Names: LAR; LAR

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity.