Mouse anti-Macaque Protein Phosphatase 1G Monoclonal Antibody | anti-PPM1G antibody

Protein Phosphatase 1G (formerly 2C), Magnesium-dependent, gamma (Protein Phosphatase Magnesium-dependent 1 gamma, PPM1G, MGC1675, MGC2870, Protein Phosphatase 1C, Protein Phosphatase 2 Catalytic Subunit gamma Isoform, Protein phosphatase 2C gamma isoform

Cat. Num. AAA606347

• Gene Names: PPM1G; PP2CG; PPP2CG; MGC1675; MGC2870; PP2CGAMMA
• Reactivity: Macaque
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G immunoaffinity chromatography.
• Synonyms: Protein Phosphatase 1G; Monoclonal Antibody; Protein Phosphatase 1G (formerly 2C); Magnesium-dependent; gamma (Protein Phosphatase Magnesium-dependent 1 gamma; PPM1G; MGC1675; MGC2870; Protein Phosphatase 1C; Protein Phosphatase 2 Catalytic Subunit gamma Isoform; Protein phosphatase 2C gamma isoform; Anti -Protein Phosphatase 1G (formerly 2C); anti-PPM1G antibody

• Host: Mouse
• Reactivity: Macaque
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9H174
• Specificity: Detects endogenous human PP2C at 75kD using Western blot.
• Purity/Purification: Affinity Purified; Purified by Protein G immunoaffinity chromatography.
• Form/Format: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.

• Applicable Applications for anti-PPM1G antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.25ug/ml; IOptimal dilutions to be determined by the researcher.
• Immunogen: Purified, E. coli-derived full length recombinant human PP2Cgamma (rhPP2Cgamma; aa 1-546; Accession# O15355).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPM1G antibody

Protein phosphatase 2C isoform gamma (PP2C gamma; also PPM1G) is a widely expressed, 75kD member of the PP2C family of Ser/Thr protein phosphatases. Human PP2C gamma is 546 amino acids (aa) in length. The apparent mass is much larger than the predicted mass of 59kD because of the presence of an acidic region (aa 259-326) rich in glutamate and aspartate residues, causing the protein to move anomalously on SDS-PAGE. The protein contains numerous sites for phosphorylation by casein kinase II, particularly within the acidic domain.

Product Categories/Family for anti-PPM1G antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 29826282
• NCBI GeneID: 5496
• NCBI Accession #: NP_817092.1
• NCBI GenBank Nucleotide #: NM_177983.1
• UniProt Accession #: O15355; Q59GB2; Q6IAU5; Q6PJK3; Q96IN7
• Molecular Weight: 59,272 Da
• NCBI Official Full Name: protein phosphatase 1G
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1G
• NCBI Official Symbol: PPM1G
• NCBI Official Synonym Symbols: PP2CG; PPP2CG; MGC1675; MGC2870; PP2CGAMMA
• NCBI Protein Information: protein phosphatase 1G; PP2C-gamma; PP2C, gamma; OTTHUMP00000123426; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• UniProt Protein Name: Protein phosphatase 1G
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1G
• UniProt Synonym Gene Names: PPM1C
• UniProt Entry Name: PPM1G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq]

Uniprot Description

PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Spliceosome

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; cell cycle arrest