Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat F2R/PAR1 Polyclonal Antibody | anti-F2R antibody
Goat anti-F2R/PAR1 Antibody
Western Blot: Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
Uniprot Description
PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.
Protein type: Cell development/differentiation; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 5q13
Cellular Component: caveola; cell surface; cytosol; early endosome; extracellular region; Golgi apparatus; integral to plasma membrane; late endosome; neuromuscular junction; plasma membrane; postsynaptic membrane
Molecular Function: G-protein alpha-subunit binding; G-protein beta-subunit binding; G-protein coupled receptor activity; protein binding; receptor binding; thrombin receptor activity
Biological Process: activation of MAPKK activity; anatomical structure morphogenesis; blood coagulation; caspase activation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); establishment of synaptic specificity at neuromuscular junction; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); homeostasis of number of cells within a tissue; inflammatory response; negative regulation of cell proliferation; negative regulation of glomerular filtration; negative regulation of neuron apoptosis; platelet activation; platelet dense granule organization and biogenesis; positive regulation of blood coagulation; positive regulation of calcium ion transport; positive regulation of caspase activity; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of Rho protein signal transduction; positive regulation of smooth muscle contraction; positive regulation of transcription, DNA-dependent; positive regulation of vasoconstriction; protein kinase C activation; regulation of blood coagulation; regulation of interleukin-1 beta production; regulation of sensory perception of pain; release of sequestered calcium ion into cytosol; response to lipopolysaccharide; response to wounding; STAT protein nuclear translocation; tyrosine phosphorylation of STAT protein
Research Articles on F2R
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Product Notes
The F2R f2r (Catalog #AAA422062) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-F2R/PAR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's F2R/PAR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the F2R f2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TRARRPESKA TNAT. It is sometimes possible for the material contained within the vial of "F2R/PAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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