Mouse anti-Human Progesterone Receptor Monoclonal Antibody | anti-PGR antibody

Progesterone Receptor (NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B) (AP)

Cat. Num. AAA6124462

• Gene Names: PGR; PR; NR3C3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Progesterone Receptor; Monoclonal Antibody; Progesterone Receptor (NR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRA; PRB; Progesterone receptor form A; Progesterone receptor form B) (AP); NR3C3; Progesterone receptor form B; anti-PGR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM566
• Specificity: Recognizes human Progesterone Receptor.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PGR antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 0.5-1ug/ml for 2 hours at RT; IHC: 0.5-1ug/ml for 30 min at RT (IHC recommended); Applications are based on unconjugated antibody.
• Immunogen: Recombinant human Progesterone Receptor protein. Cellular Localization: Nuclear
• Conjugate: AP
• Positive Control: T47-D Cells or Breast Cancers.
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.

Immunohistochemistry (IHC)

(Formalin-paraffin normal human breast carcinoma stained with Progesterone Receptor MAb (SPM566).)

Product Categories/Family for anti-PGR antibody

Antibodies; Receptors

References

1. Press M, et al. Steroids. 2002 Aug; 67(9):799-813. 2. Mote P, et al. J Clin Pathol., 2001; 54: 624-630.

NCBI and Uniprot Product Information

• NCBI GI #: 110611914
• NCBI GeneID: 5241
• NCBI Accession #: NP_000917.3
• NCBI GenBank Nucleotide #: NM_000926.4
• UniProt Accession #: P06401; Q9UPF7; A7X8B0
• Molecular Weight: 98,981 Da
• NCBI Official Full Name: progesterone receptor isoform B
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor; nuclear receptor subfamily 3 group C member 3
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR
• UniProt Entry Name: PRGR_HUMAN

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]

Uniprot Description

PR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; mitochondrial outer membrane

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; steroid hormone receptor activity; steroid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; progesterone receptor signaling pathway; cell-cell signaling; epithelial cell maturation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction; ovulation from ovarian follicle; regulation of epithelial cell proliferation

Disease: Progesterone Resistance