Mouse PRMT6 Monoclonal Antibody | anti-PRMT6 antibody

PRMT6 (Protein Arginine Methyltransferase 6, FLJ10559, HRMT1L6) (AP)

Cat. Num. AAA6165522

• Applications: Western Blot
• Purity: Purified
• Synonyms: PRMT6; Monoclonal Antibody; PRMT6 (Protein Arginine Methyltransferase 6; FLJ10559; HRMT1L6) (AP); Protein Arginine Methyltransferase 6; HRMT1L6; anti-PRMT6 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3C3
• Specificity: Recognizes PRMT6.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 316

• Applicable Applications for anti-PRMT6 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PRMT6 (NP_060607.1, 1aa-316aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PRMT6 monoclonal antibody (M02), clone 3C3. Western Blot analysis of PRMT6 expression in MCF-7.)

Testing Data

(Detection limit for recombinant GST tagged PRMT6 is 0.1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of PRMT6 expression in transfected 293T cell line by PRMT6 monoclonal antibody (M02), clone 3C3.Lane 1: PRMT6 transfected lysate (Predicted MW: 35.2 KDa).Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PRMT6 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PRMT6 on HeLa cell. [antibody concentration 10 ug/ml])

Related Product Information for anti-PRMT6 antibody

Protein arginine N-methyltransferases, such as PRMT6, catalyze the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. [supplied by OMIM]

Product Categories/Family for anti-PRMT6 antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 8922515
• NCBI Accession #: NP_060607.1
• NCBI GenBank Nucleotide #: NP_060607.1
• NCBI Official Full Name: protein arginine methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase