Western Blot (WB)
(PRKAA1 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PRKAA1 antibody detected the PRKAA1 protein (arrow).)
Mouse anti-Human PRKAA1 Monoclonal Antibody | anti-PRKAA1 antibody
PRKAA1 (PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1) (APC)
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
PRKAA1; Monoclonal Antibody; PRKAA1 (PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase; Tau-protein kinase PRKAA1) (APC); Tau-protein kinase PRKAA1; anti-PRKAA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
167CT22.1.6
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PRKAA1 antibody
FLISA, Western Blot (WB)
Application Notes
FLISA: 1:1000
WB: 1:100-500
Applications are based on unconjugated antibody.
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PRKAA1 Mab is generated from mouse immunized with PRKAA1 recombinant protein.
Conjugate
APC
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze APC conjugates. Light sensitive.
Western Blot (WB)
(PRKAA1 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PRKAA1 antibody detected the PRKAA1 protein (arrow).)
Western Blot (WB)
(PRKAA1 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PRKAA1 antibody detected the PRKAA1 protein (arrow).)
Related Product Information for anti-PRKAA1 antibody
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Product Categories/Family for anti-PRKAA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63kDa
NCBI Official Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
Similar Products
Product Notes
The PRKAA1 prkaa1 (Catalog #AAA6121056) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRKAA1 (PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAA1 can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). FLISA: 1:1000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRKAA1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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