Mouse anti-Mammalian Cytokeratin 18 Monoclonal Antibody | anti-KRT18 antibody

Anti-Cytokeratin 18 Alexa Fluor488

• Gene Names: KRT18; K18; CYK18
• Reactivity: Mammalian
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: Cytokeratin 18; Monoclonal Antibody; Anti-Cytokeratin 18 Alexa Fluor488; K18; CK18; CYK18; KRT18; keratin 18; anti-KRT18 antibody

• Host: Mouse
• Reactivity: Mammalian
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: C-04
• Specificity: The antibody C-04 reacts with Cytokeratin 18 (45kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-KRT18 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 3-12ug/ml. Intracellular staining.
• Conjugation: Alexa Fluor488
• Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (intracellular staining) of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) Alexa Fluor 488.)

Related Product Information for anti-KRT18 antibody

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40kDa and 68kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).

References

*Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834dopt=A,*Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228dopt=A,*Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504., URL: https://www.ncbi.nlm.nih.gov/pubmed/2461901dopt=A,*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305dopt=A

NCBI and Uniprot Product Information

• NCBI GI #: 4557888
• NCBI GeneID: 3875
• NCBI Accession #: NP_000215.1
• NCBI GenBank Nucleotide #: NM_000224.2
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 18
• NCBI Official Synonym Full Names: keratin 18
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• Protein Family: Keratin
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; CK-18; K18
• UniProt Entry Name: K1C18_HUMAN

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; microtubule organizing center; intermediate filament

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial