Mouse anti-Human PRDX3 Monoclonal Antibody | anti-PRDX3 antibody

PRDX3 (Thioredoxin-dependent Peroxide Reductase, Mitochondrial, Antioxidant Protein 1, AOP-1, HBC189, Peroxiredoxin III, Prx-III, Peroxiredoxin-3, Protein MER5 Homolog, AOP1, MER5, MGC104387, MGC24293, PRO1748) (HRP)

Cat. Num. AAA6154326

• Gene Names: PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRDX3; Monoclonal Antibody; PRDX3 (Thioredoxin-dependent Peroxide Reductase; Mitochondrial; Antioxidant Protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 Homolog; AOP1; MER5; MGC104387; MGC24293; PRO1748) (HRP); anti-PRDX3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1E3-B2
• Specificity: Recognizes human PRDX3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PRDX3 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-257 from human PRDX3 (AAH02685) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody Lane 1: PRDX3 transfected lysate (27.7kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PRDX3 on HeLa cell. [antibody concentration 10ug/ml].)

Immunoprecipitation (IP)

(Immunoprecipitation of PRDX3 transfected lysate using PRDX3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with PRDX3 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged PRDX3 is ~1ng/ml as a capture antibody.)

Related Product Information for anti-PRDX3 antibody

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Product Categories/Family for anti-PRDX3 antibody

Antibodies; Enzymes; Peroxiredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 12803698
• NCBI GeneID: 10935
• Molecular Weight: 25,839 Da
• NCBI Official Full Name: Homo sapiens peroxiredoxin 3, mRNA
• NCBI Official Synonym Full Names: peroxiredoxin 3
• NCBI Official Symbol: PRDX3
• NCBI Official Synonym Symbols: AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• NCBI Protein Information: thioredoxin-dependent peroxide reductase, mitochondrial
• Protein Family: Thioredoxin-dependent peroxide reductase

NCBI Description

This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]