Testing Data
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Mouse anti-Broad Polyubiquitin Monoclonal Antibody | anti-MubC5 antibody
Mouse anti Polyubiquitin (K63-LINKAGE Specific)
Gene Names
MubC5; MUB14; ubiquitin; ubiquitin2; GRMZM2G419891
Reactivity
Broad
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Purified by ion exchange chromatography from tissue culture supernatant
Synonyms
Polyubiquitin; Monoclonal Antibody; Mouse anti Polyubiquitin (K63-LINKAGE Specific); Polyubiquitin (K63-LINKAGE Specific) Antibody; anti-MubC5 antibody
Host
Mouse
Reactivity
Broad
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HWA4C4
Purity/Purification
Purified by ion exchange chromatography from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
533
Applicable Applications for anti-MubC5 antibody
Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC: 1:100
IHC-Formalin: 1:100
WB: 1:500-1:1000
IHC-Formalin: 1:100
WB: 1:500-1:1000
Immunogen
Synthetic peptide containing the ubiquitin K63-isopeptide motif
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Testing Data
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Testing Data
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Related Product Information for anti-MubC5 antibody
Mouse anti polyubiquitin (K63-linkage specific) antibody clone HWA4C4 recognizes Lysine-63 linked polyubiquitin chains.
Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. The protein is covalently linked to selected lysine residues in a post-translational modification process known as ubiquitylation or ubiquitination. This chemical reaction is mediated by three different protein families; ubiquitin-activating enzymes (also known as E1s), ubiquitin-conjugating enzymes (also known as E2s) and ubiquitin ligases (also known as E3s) (Hershko and Ciechanover 1998).
The impact of ubiquitination depends on whether a single ubiquitin moiety (monoubiquitination) or an ubiquitin chain (polyubiquitination) has been attached to a protein. Monoubiquitination tends to trigger cellular processes related to endocytosis and membrane trafficking (Haglund et al. 2003) while the impact of polyubiquitination varies depending on how the ubiquitin residues in the chain have been linked. Attachment of Lysine-48 ubiquitin chains results in degradation by the 20S proteasome while addition of Lysine-63 ubiquitin chains mediates DNA damage and NFkappaB signaling (Chen 2005; Mocciaro and Rape 2012). Lysine-6, Lysine-11, Lysine-27, Lysine-29 and Lysine-33 chains have also been reported (Komander 2009 and Ye and Rape 2009).
Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. The protein is covalently linked to selected lysine residues in a post-translational modification process known as ubiquitylation or ubiquitination. This chemical reaction is mediated by three different protein families; ubiquitin-activating enzymes (also known as E1s), ubiquitin-conjugating enzymes (also known as E2s) and ubiquitin ligases (also known as E3s) (Hershko and Ciechanover 1998).
The impact of ubiquitination depends on whether a single ubiquitin moiety (monoubiquitination) or an ubiquitin chain (polyubiquitination) has been attached to a protein. Monoubiquitination tends to trigger cellular processes related to endocytosis and membrane trafficking (Haglund et al. 2003) while the impact of polyubiquitination varies depending on how the ubiquitin residues in the chain have been linked. Attachment of Lysine-48 ubiquitin chains results in degradation by the 20S proteasome while addition of Lysine-63 ubiquitin chains mediates DNA damage and NFkappaB signaling (Chen 2005; Mocciaro and Rape 2012). Lysine-6, Lysine-11, Lysine-27, Lysine-29 and Lysine-33 chains have also been reported (Komander 2009 and Ye and Rape 2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
polyubiquitin
NCBI Official Symbol
MubC5
NCBI Official Synonym Symbols
MUB14; ubiquitin; ubiquitin2; GRMZM2G419891
NCBI Protein Information
polyubiquitin
Protein Family
Similar Products
Product Notes
The MubC5 (Catalog #AAA225371) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Polyubiquitin (K63-LINKAGE Specific) reacts with Broad and may cross-react with other species as described in the data sheet. AAA Biotech's Polyubiquitin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1:100 IHC-Formalin: 1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MubC5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Polyubiquitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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