Mouse anti-Human PMS2 (Postmeiotic Segregation Increased 2) Monoclonal Antibody | anti-PMS2 antibody

PMS2 (Postmeiotic Segregation Increased 2)

Cat. Num. AAA556114

• Gene Names: PMS2; PMSL2; HNPCC4; PMS2CL
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: PMS2 (Postmeiotic Segregation Increased 2); Monoclonal Antibody; anti-PMS2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Form/Format: Affinity Purified antibody; Liquid with PBS buffer, pH7.4 with 0.02% Sodium Azide

• Applicable Applications for anti-PMS2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:1,000-3,000
• Immunogen: Recombinant Human PMS2
• Preparation and Storage: The product can be stored undiluted for several weeks at 4 degree C. Dilute only immediately before use. Aliquot and store at -20 degree C for long term. Avoid freeze thaw-cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry staining of MO47015 on human cervix tissue.)

Immunohistochemistry (IHC)

(Immunohistochemistry staining of MO47015 on human cervix tissue.)

Related Product Information for anti-PMS2 antibody

PMS2 (Postmeiotic Segregation Increased 2) is a DNA repair protein involved in mismatch repair. PMS2 Antibody was developed alongsidemlH1, MSH2, and MSH6 to complete our MMR-series for research use. PMS2 expression levels may be used theorectically as a screening tool for Lynch syndrome after a colorectal cancer identification.

NCBI and Uniprot Product Information

• NCBI GI #: 4505913
• NCBI GeneID: 5395
• NCBI Accession #: NP_000526.1
• NCBI GenBank Nucleotide #: NM_000535.5
• UniProt Accession #: P54278; Q52LH6; Q5FBW9; Q5FBX1; Q5FBX2; Q75MR2; B2R610
• Molecular Weight: 95,797 Da
• NCBI Official Full Name: mismatch repair endonuclease PMS2 isoform a
• NCBI Official Synonym Full Names: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• NCBI Official Symbol: PMS2
• NCBI Official Synonym Symbols: PMSL2; HNPCC4; PMS2CL
• NCBI Protein Information: mismatch repair endonuclease PMS2; H_DJ0042M02.9; PMS1 protein homolog 2; DNA mismatch repair protein PMS2
• UniProt Protein Name: Mismatch repair endonuclease PMS2
• Protein Family: Mismatch repair endonuclease
• UniProt Gene Name: PMS2
• UniProt Synonym Gene Names: PMSL2
• UniProt Entry Name: PMS2_HUMAN

NCBI Description

This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PMS2: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS); also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots. Belongs to the DNA mismatch repair MutL/HexB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.1.-.-; Hydrolase; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22.2

Cellular Component: MutLalpha complex; microtubule cytoskeleton; nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; single base insertion or deletion binding; endonuclease activity; ATPase activity; MutSalpha complex binding; single-stranded DNA binding; ATP binding

Biological Process: response to drug; mismatch repair; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; DNA repair

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 4; Mismatch Repair Cancer Syndrome