Mouse anti-Human PLA2G4B Monoclonal Antibody | anti-PLA2G4B antibody

PLA2G4B (Cytosolic Phospholipase A2 beta, cPLA2-beta, Phospholipase A2 Group IVB) (AP)

Cat. Num. AAA6132989

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLA2G4B; Monoclonal Antibody; PLA2G4B (Cytosolic Phospholipase A2 beta; cPLA2-beta; Phospholipase A2 Group IVB) (AP); anti-PLA2G4B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1G5
• Specificity: Recognizes human PLA2G4B.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PLA2G4B antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-317 from PLA2G4B (AAH25290) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLPYFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELPQLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYELYTPATYQLTEEGTFKVVDEEAMEKVPWIPLDPLAPDLARYPSYSQ
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PLA2G4B is ~3ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of PLA2G4B expression in transfected 293T cell line by PLA2G4B monoclonal antibody Lane 1: PLA2G4B transfected lysate (114.121kD). Lane 2: Non-transfected lysate.)

Western Blot (WB)

(Western Blot detection against Immunogen (60.87kD).)

Related Product Information for anti-PLA2G4B antibody

This gene transcribes naturally-occurring mRNAs that are co-transcribed products of the neighboring JMJD7 and PLA2G4B genes. Incompletely processed read-through transcripts from these two loci are abundantly expressed in most tissues, but the function of the predicted protein product has not yet been determined. Alternative splicing of this gene results in different transcript variants, some of which are candidates for nonsense-mediated decay (NMD).

Product Categories/Family for anti-PLA2G4B antibody

Antibodies; Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 19263690
• NCBI GeneID: 100137047
• Molecular Weight: 35,932 Da
• NCBI Official Full Name: Homo sapiens jumonji domain containing 7, mRNA
• NCBI Official Synonym Full Names: jumonji domain containing 7
• NCBI Official Symbol: JMJD7
• NCBI Protein Information: jmjC domain-containing protein 7
• Protein Family: Cytosolic phospholipase

NCBI Description

This gene encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein. [provided by RefSeq, Jul 2008]