Mouse anti-Human, rat Piccolo Monoclonal Antibody
Anti-Piccolo Monoclonal Antibody
Reactivity
Human, rat
Applications
ELISA, Immunoblot, Western Blot
Synonyms
Piccolo; Monoclonal Antibody; Anti-Piccolo Monoclonal Antibody; anti-Piccolo antibody
Host
Mouse
Reactivity
Human, rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6H9-B6
Specificity
Piccolo
This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.
This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.
Form/Format
100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Applicable Applications for anti-Piccolo antibody
ELISA; Immunoblot
Application Notes
Immunoblotting: use at 1-10ug/ml. A band of ~550kDa is detected. ELISA: use at 1-10ug/ml with Piccolo on the solid phase. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.
Antigen
Full-length human Piccolo protein (accession no. BC001304.1)
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-Piccolo antibody
Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin-mediated endocytosis by binding to the F-actin and dynamin bind protein Abp1.
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Product Notes
The Piccolo (Catalog #AAA190592) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Piccolo Monoclonal Antibody reacts with Human, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Piccolo can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot. Immunoblotting: use at 1-10ug/ml. A band of ~550kDa is detected. ELISA: use at 1-10ug/ml with Piccolo on the solid phase. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate. Researchers should empirically determine the suitability of the Piccolo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Piccolo, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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