Testing Data
Mouse NR2B Glutamate Receptor Monoclonal Antibody
Anti-NR2B Glutamate Receptor Monoclonal Antibody
Reactivity
Human, mouse, rat
Applications
Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
NR2B Glutamate Receptor; Monoclonal Antibody; Anti-NR2B Glutamate Receptor Monoclonal Antibody; anti-NR2B Glutamate Receptor antibody
Host
Mouse
Reactivity
Human, mouse, rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S59-20
Specificity
NR2B Glutamate Receptor
This antibody recognizes human, mouse, and rat NR2B. It does not cross-react with NR2A.
This antibody recognizes human, mouse, and rat NR2B. It does not cross-react with NR2A.
Form/Format
100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Sequence Positions
20-271
Applicable Applications for anti-NR2B Glutamate Receptor antibody
Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
Application Notes
Immunoblotting: use at 1-10ug/ml. A band of ~166kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate
Antigen
Fusion protein aa 20-271 (extracellular N-terminus) of rat NR2B (accession no. Q00960). This sequence is 99% identical to human and mouse.
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Testing Data
Testing Data
Related Product Information for anti-NR2B Glutamate Receptor antibody
Glutamate receptors of the NMDA subtype are involved in several physiological and pathological processes in the brain. Functional NMDA receptors are heteromultimeric complexes of the NR1 subunit and one or more of the four NR2 subunits (NR2A-D). Coexpression of both subunit types are required for the formation of fully functional channels that are inserted into the plasma membrane. The NR2 subunit determines characteristics of the channel such as conductance, mean open time, and sensitivity to Mg+2. The ability of NR1 and NR2 subunit combinations to flux Ca+2 is essential to the role that NMDA receptors play in synaptic plasticity and neurotoxicity.
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Product Notes
The NR2B Glutamate Receptor (Catalog #AAA190553) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-271. The Anti-NR2B Glutamate Receptor Monoclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's NR2B Glutamate Receptor can be used in a range of immunoassay formats including, but not limited to, Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence. Immunoblotting: use at 1-10ug/ml. A band of ~166kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate. Researchers should empirically determine the suitability of the NR2B Glutamate Receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2B Glutamate Receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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