Rabbit PI3K p55gamma Monoclonal Antibody | anti-PI3K antibody

PI3K p55gamma Rabbit Monoclonal Antibody

Cat. Num. AAA3014077

• Gene Names: PIK3R3; p55; p55PIK; p55-GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Protein A affinity purified
• Synonyms: PI3K p55gamma; Monoclonal Antibody; PI3K p55gamma Rabbit Monoclonal Antibody; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK; PIK3R3; anti-PI3K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of PI3K p55gamma and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 461

• Applicable Applications for anti-PI3K antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:10,000; ICC: 1:50-1:200; IF: 1:50-1:200; IHC: 1:200-1:500
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PI3K p55gamma Rabbit mAb at 1:1000 dilutionLane1:MCF7 whole cell lysateLane2:Jurkat whole cell lysateLane3:The testis tissue lysate of Mouse)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 gamma antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PI 3 Kinase p55 gamma in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 gamma antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PI 3 Kinase p55 gamma in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Related Product Information for anti-PI3K antibody

Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3 position. PI 3-kinase p55gamma (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55gamma protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55gamma mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55gamma have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55gamma interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 317373310
• NCBI GeneID: 8503
• UniProt Accession #: Q92569
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 3
• NCBI Official Symbol: PIK3R3
• NCBI Official Synonym Symbols: p55; p55PIK; p55-GAMMA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit gamma
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• UniProt Gene Name: PIK3R3
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma
• UniProt Entry Name: P55G_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016]

Uniprot Description

PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity

Biological Process: phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; regulation of phosphoinositide 3-kinase activity