Rabbit PI3K p55gamma Polyclonal Antibody | anti-PI3K antibody

PI3K p55gamma (Y463) Polyclonal Antibody

Cat. Num. AAA3003307

• Gene Names: PIK3R3; p55; p55PIK; p55-GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PI3K p55gamma; Polyclonal Antibody; PI3K p55gamma (Y463) Polyclonal Antibody; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK; PIK3R3; anti-PI3K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PI3K p55gamma (Y463) polyclonal antibody detects endogenous levels of PI3-kinase p85alpha protein. This antibody also detects PI3K p55gamma protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 461

• Applicable Applications for anti-PI3K antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 430-480 of Human PI3K p85alpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PI3K p85alpha/p55gamma (Y463) pAb at 1:500 dilutionLane1:HCC827 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PI3K p85 alpha/gamma (Y463) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-PI3K antibody

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 317373310
• NCBI GeneID: 8503
• UniProt Accession #: Q92569
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 3
• NCBI Official Symbol: PIK3R3
• NCBI Official Synonym Symbols: p55; p55PIK; p55-GAMMA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit gamma
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• UniProt Gene Name: PIK3R3
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma
• UniProt Entry Name: P55G_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016]

Uniprot Description

PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity

Biological Process: phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; regulation of phosphoinositide 3-kinase activity