Human FGFRL1 ELISA Kit | FGFRL1 elisa kit

Human FGFRL1 PicoKine ELISA Kit

Cat. Num. AAA1752684

• Gene Names: FGFRL1; FHFR; FGFR5; FGFR-5
• Reactivity: Human
• Synonyms: FGFRL1; Human FGFRL1 PicoKine ELISA Kit; Human FGFRL1; fibroblast growth factor receptor like 1; Fibroblast growth factor receptor-like 1; FGF receptor-like protein 1; FGF homologous factor receptor; FGFR-like protein; Fibroblast growth factor receptor 5; FGFR-5; FGFR5; FHFR; UNQ480; PRO943; FGFRL1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human FGFRL1
• Sequence Length: 504

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGFRL1 elisa kit

Principle of the Assay: The Picokine™ Human FGFRL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FGFRL1 with a 96-well strip plate that is pre-coated with antibody specific for FGFRL1. The detection antibody is a biotinylated antibody specific for FGFRL1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human FGFRL1 with immunogen: Expression system for standard: NSO, Immunogen sequence: A25-P378. The kit is analytically validated with ready to use reagents. To measure Human FGFRL1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FGFRL1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FGFRL1 in the sample.!!Background/Introduction: Fibroblast growth factor receptor-like 1 is a protein that in humans is encoded by the FGFRL1 gene. It is mapped to 4p16.3. The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membranespanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 51988912
• NCBI GeneID: 53834
• NCBI Accession #: NP_001004356.1
• NCBI GenBank Nucleotide #: NP_001004356.1
• UniProt Accession #: Q8N441
• NCBI Official Full Name: fibroblast growth factor receptor-like 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor like 1
• NCBI Official Symbol: FGFRL1
• NCBI Official Synonym Symbols: FHFR; FGFR5; FGFR-5
• NCBI Protein Information: fibroblast growth factor receptor-like 1
• UniProt Protein Name: Fibroblast growth factor receptor-like 1
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFRL1
• UniProt Synonym Gene Names: FGFR5; FHFR; FGF receptor-like protein 1; FGFR-5
• UniProt Entry Name: FGRL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FGFRL1: Has a negative effect on cell proliferation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi apparatus; transport vesicle; plasma membrane; integral to membrane

Molecular Function: heparin binding; fibroblast growth factor binding; fibroblast growth factor receptor activity

Biological Process: negative regulation of cell proliferation; fibroblast growth factor receptor signaling pathway; protein heterooligomerization; skeletal development; protein homooligomerization

Disease: Wolf-hirschhorn Syndrome