Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) ()

Mouse Peroxiredoxin III Monoclonal Antibody | anti-Prdx3 antibody

Peroxiredoxin III antibody

Gene Names
Prdx3; Aop1; Ef2l; Mer5; Prx3; SP22; TDXM; D0Tohi1; AW822249
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Peroxiredoxin III antibody was purified by affinity chromatography.
Synonyms
Peroxiredoxin III; Monoclonal Antibody; Peroxiredoxin III antibody; Monoclonal Peroxiredoxin III; Anti-Peroxiredoxin III; anti-Prdx3 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
4G10
Purity/Purification
Peroxiredoxin III antibody was purified by affinity chromatography.
Form/Format
0.15M Nacl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. With 0.03% sodium azide added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
257
Applicable Applications for anti-Prdx3 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 1:1000
IP1-2ul/400ul cell lysates
Biological Significance
Peroxiredoxin is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prxsubgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 ?30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI incytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol (1).
Cross-Reactivity
Human
Immunogen
Peroxiredoxin III antibody was raised in Mouse using recombinant human protein purified from E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles..

Western Blot (WB)

()

Western Blot (WB) ()

Western Blot (WB)

()

Western Blot (WB) ()

Western Blot (WB)

()

Western Blot (WB) ()
Related Product Information for anti-Prdx3 antibody
Mouse monoclonal Peroxiredoxin III antibody
Product Categories/Family for anti-Prdx3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
28,127 Da
NCBI Official Full Name
peroxiredoxin III
NCBI Official Synonym Full Names
peroxiredoxin 3
NCBI Official Symbol
Prdx3
NCBI Official Synonym Symbols
Aop1; Ef2l; Mer5; Prx3; SP22; TDXM; D0Tohi1; AW822249
NCBI Protein Information
thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Protein Name
Thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Gene Name
Prdx3
UniProt Synonym Gene Names
Aop1; Mer5; AOP-1
UniProt Entry Name
PRDX3_MOUSE

Uniprot Description

PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.

Protein type: Oxidoreductase; EC 1.11.1.15

Cellular Component: mitochondrion; early endosome; cytoplasm; IkappaB kinase complex; cytosol; myelin sheath

Molecular Function: antioxidant activity; protein C-terminus binding; identical protein binding; peroxidase activity; peroxiredoxin activity; thioredoxin peroxidase activity; caspase inhibitor activity; oxidoreductase activity; protein kinase binding; kinase binding

Biological Process: mitochondrion organization and biogenesis; negative regulation of kinase activity; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; peptidyl-cysteine oxidation; regulation of mitochondrial membrane potential; response to hydrogen peroxide; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of neuron apoptosis; maternal placenta development; negative regulation of apoptosis

Research Articles on Prdx3

Similar Products

Product Notes

The Prdx3 prdx3 (Catalog #AAA832063) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Peroxiredoxin III can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). WB: 1:1000 IP1-2ul/400ul cell lysates. Researchers should empirically determine the suitability of the Prdx3 prdx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin III, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.