Mouse Peroxiredoxin IV Monoclonal Antibody | anti-PRDX4 antibody

Peroxiredoxin IV antibody

Cat. Num. AAA833757

• Gene Names: PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
• Applications: Immunoprecipitation
• Purity: Peroxiredoxin IV antibody was purified by affinity chromatography.
• Synonyms: Peroxiredoxin IV; Monoclonal Antibody; Peroxiredoxin IV antibody; Monoclonal Peroxiredoxin IV; Anti-Peroxiredoxin IV; anti-PRDX4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 9D2
• Purity/Purification: Peroxiredoxin IV antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer (without mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-PRDX4 antibody: Immunoprecipitation (IP)
• Application Notes: IP 1-2ul/400ul lysates
• Biological Significance: Peroxiredoxin is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prxsubgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 ?30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI incytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol (1).
• Cross-Reactivity: Human
• Immunogen: Peroxiredoxin IV antibody was raised in Mouse using recombinant human protein purified from E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles..

Immunofluorescence (IF)

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Western Blot (WB)

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Related Product Information for anti-PRDX4 antibody

Mouse monoclonal Peroxiredoxin IV antibody

Product Categories/Family for anti-PRDX4 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 5453549
• NCBI GeneID: 10549
• NCBI Accession #: NP_006397.1
• NCBI GenBank Nucleotide #: NM_006406.1
• UniProt Accession #: Q13162; Q6FHT3
• Molecular Weight: 30,540 Da
• NCBI Official Full Name: peroxiredoxin-4
• NCBI Official Synonym Full Names: peroxiredoxin 4
• NCBI Official Symbol: PRDX4
• NCBI Official Synonym Symbols: PRX-4; AOE372; AOE37-2; HEL-S-97n
• NCBI Protein Information: peroxiredoxin-4
• UniProt Protein Name: Peroxiredoxin-4
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX4
• UniProt Synonym Gene Names: AOE37-2; Prx-IV
• UniProt Entry Name: PRDX4_HUMAN

NCBI Description

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family.

Protein type: Secreted; Mitochondrial; Secreted, signal peptide; EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; smooth endoplasmic reticulum; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; thioredoxin peroxidase activity

Biological Process: protein maturation via protein folding; extracellular matrix organization and biogenesis; 4-hydroxyproline metabolic process; cell redox homeostasis; male gonad development; spermatogenesis; I-kappaB phosphorylation