Human Small ubiquitin related modifier 2 (SUMO2) ELISA Kit | SUMO2 elisa kit

Human Small ubiquitin related modifier 2 (SUMO2) ELISA Kit

Cat. Num. AAA7231157

• Gene Names: Sumo2; Smt3A; Smt3h2
• Reactivity: Human
• Synonyms: Small ubiquitin related modifier 2 (SUMO2); Human Small ubiquitin related modifier 2 (SUMO2) ELISA Kit; SUMO2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for SUMO2 elisa kit

Intended Uses: This SUMO2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SUMO2. This ELISA kit for research use only!

Principle of the Assay: SUMO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SUMO2 antibody and an SUMO2-HRP conjugate. The assay sample and buffer are incubated together with SUMO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SUMO2 concentration since SUMO2 from samples and SUMO2-HRP conjugate compete for the anti-SUMO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SUMO2 from the sample, fewer sites are left to bind SUMO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SUMO2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19424298
• NCBI GeneID: 690244
• NCBI Accession #: NP_598278.1
• NCBI GenBank Nucleotide #: NM_133594.2
• Molecular Weight: 10,871 Da
• NCBI Official Full Name: small ubiquitin-related modifier 2
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: Sumo2
• NCBI Official Synonym Symbols: Smt3A; Smt3h2
• NCBI Protein Information: small ubiquitin-related modifier 2; SUMO-2; sentrin-2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: Sumo2
• UniProt Synonym Gene Names: Smt3a; Smt3h2; SUMO-2; Smt3A
• UniProt Entry Name: SUMO2_RAT

NCBI Description

LDH isozyme which show evolutionary relationships among vertebrate [RGD, Feb 2006]

Uniprot Description

SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Cellular Component: PML body; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; SUMO ligase activity

Biological Process: protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent