Mouse PDLIM2 Monoclonal Antibody | anti-PDLIM2 antibody

PDLIM2 antibody

Cat. Num. AAA834991

• Gene Names: PDLIM2; SLIM; MYSTIQUE
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: PDLIM2 antibody was purified by affinity chromatography.
• Synonyms: PDLIM2; Monoclonal Antibody; PDLIM2 antibody; Monoclonal PDLIM2; Anti-PDLIM2; PDZ and LIM domain 2; PDZ-LIM protein mystique; MYSTIQUE; FLJ34715; SLIM; PDLIM 2; PDLIM-2; anti-PDLIM2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 15C1
• Purity/Purification: PDLIM2 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 602

• Applicable Applications for anti-PDLIM2 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:500-2000
• Biological Significance: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.
• Cross-Reactivity: Human
• Immunogen: PDLIM2 antibody was raised in mouse using a full length recombinant protein of human PDLIM2 (NP_067643) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using PDLIM2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDLIM2 protein (Right) or empty vector (Left) detected with PDLIM2 antibody)

Related Product Information for anti-PDLIM2 antibody

Mouse monoclonal PDLIM2 antibody

Product Categories/Family for anti-PDLIM2 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 311771772
• NCBI GeneID: 64236
• NCBI Accession #: NP_067643
• NCBI GenBank Nucleotide #: NM_021630.5
• UniProt Accession #: Q96JY6; Q7Z584; Q86WM8; Q8WZ29; Q9H4L9; Q9H7I2; D3DSR5; J3KNH4
• Molecular Weight: 62,746 Da
• NCBI Official Full Name: PDZ and LIM domain protein 2 isoform 2
• NCBI Official Synonym Full Names: PDZ and LIM domain 2 (mystique)
• NCBI Official Symbol: PDLIM2
• NCBI Official Synonym Symbols: SLIM; MYSTIQUE
• NCBI Protein Information: PDZ and LIM domain protein 2
• UniProt Protein Name: PDZ and LIM domain protein 2
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM2
• UniProt Entry Name: PDLI2_HUMAN

NCBI Description

This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PDLIM2: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cortical actin cytoskeleton; focal adhesion; stress fiber; nucleus; actin cytoskeleton

Molecular Function: zinc ion binding; filamin binding; muscle alpha-actinin binding; myosin heavy chain binding