Mouse anti-Human PDL2 Monoclonal Antibody | anti-PDL2 antibody

PDL2 Antibody [7C1]

Cat. Num. AAA154568

• Gene Names: PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: PDL2; Monoclonal Antibody; PDL2 Antibody [7C1]; B7DC; Btdc; CD273; PD-L2; PDCD1L2; bA574F11.2; Programmed cell death 1 ligand 2; Butyrophilin B7-DC; PD-1 ligand 2; programmed cell death 1 ligand; anti-PDL2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 7C1
• Purity/Purification: Protein A purified
• Form/Format: Liquid; Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 273

• Applicable Applications for anti-PDL2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB at 0.5-1 ug/mL; IHC starting at 2 ug/mL; IF start at 20 ug/mL.; ; Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Immunogen: PD-L2 antibody was raised against the extracellular domain of human PD-L2.
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml)

Immunocytochemistry (IHC)

(Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [7C1]Blue: DAPI staining)

Immunofluorescence (IF)

(Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [7C1]Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL. )

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells. )

Related Product Information for anti-PDL2 antibody

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.

Product Categories/Family for anti-PDL2 antibody

Immunology

References

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78. Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

NCBI and Uniprot Product Information

• NCBI GI #: 190014605
• NCBI GeneID: 80380
• NCBI Accession #: NP_079515
• NCBI GenBank Nucleotide #: NM_025239.3
• UniProt Accession #: P9BQ51; Q14CN8; Q5T7Z6; Q6JXL8; Q6JXL9; Q9BQ51
• Molecular Weight: Predicted: 30 kDa; Observed: 38 kDa
• NCBI Official Full Name: programmed cell death 1 ligand 2
• NCBI Official Synonym Full Names: programmed cell death 1 ligand 2
• NCBI Official Symbol: PDCD1LG2
• NCBI Official Synonym Symbols: B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• NCBI Protein Information: programmed cell death 1 ligand 2
• UniProt Protein Name: Programmed cell death 1 ligand 2
• UniProt Gene Name: PDCD1LG2
• UniProt Synonym Gene Names: B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC

Uniprot Description

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production ().