Human PD-L1 DIY ELISA Kit | PD-L1 diy elisa kit

Pre-Titrated Reagents for Human PD-L1 ELISA

Cat. Num. AAA590081

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Synonyms: PD-L1; Pre-Titrated Reagents for Human PD-L1 ELISA; Pre-Titrated Reagents for Programmed Death-Ligand 1; Pre-Titrated reagents for making PD-L1 ELISA kit; PD-L1 diy elisa kit

• Reactivity: Human
• Sequence Length: 245

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PD-L1 diy elisa kit

Intended Uses: Pre-titrated Reagent Kit for human PD-L1 contains calibrated PD-L1 standards and pre-titrated capture and detection antibodies for quantitative measurement of human PD-L1 in biological samples by enzyme-linked immunoassay (ELISA). An assay protocol is provided with the kit to use the reagents in 96-well ELISA format. It is recommended to dilute all reagents according to this manual. The reagents can also be used with 384-well ELISA plate and optimized for use in other type of immunoassays.!!Background/Introduction: Programmed Death Ligand 1 (PD-L1) is a 40KD transmembrane protein that can link with immune checkpoint protein Programmed Death-1 (PD-1) when T-cell receptors engage with its specific antigen. During T cell receptor signaling, the binding of PD-L1 with PD-1 transmits an inhibitory signals to IL-2 production and T cell proliferation. This inhibitory co-stimulation plays an important role in body's immune suppressive functions, such as self-tolerance, suppression of immune-rejection during pregnancy and allograft etc. Upregulation of PD-L1 was found to be associated with many cancer types and is believed to contribute to the immune evasion by cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 930425329
• NCBI GeneID: 29126
• NCBI Accession #: NP_001300958.1
• NCBI GenBank Nucleotide #: NM_001314029.1
• Molecular Weight: 20,246 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform c
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: PD-L12 PublicationsManual assertion based on opinion iniRef.10; PDCD1 ligand 1; Programmed death ligand 1; B7-H11 PublicationManual assertion based on opinion iniRef.1

NCBI Description

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).