Western Blot (WB)
(Western blot detection of PDK2 in A549, MCF7, C6 and Hela cell lysates using PDK2 mouse mAb (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
Mouse anti-Human, Rat PDK2 Monoclonal Antibody | anti-PDK2 antibody
Anti-PDK2 Mouse mAb
Western Blot (WB)
(Western blot detection of PDK2 in A549, MCF7, C6 and Hela cell lysates using PDK2 mouse mAb (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
Western Blot (WB)
(Western blot detection of PDK2 in A549, MCF7, C6 and Hela cell lysates using PDK2 mouse mAb (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
Western Blot (WB)
(Western blot analysis using PDK2 mAb against HEK293 (1) and PDK2 (AA)
Western Blot (WB)
(Western blot analysis using PDK2 mAb against HEK293 (1) and PDK2 (AA)
Western Blot (WB)
(Western blot analysis using PDK2 mouse mAb against Jurkat (1), C6 (2), Cos7 (3), K562 (4), A431 (5) cell lysate.)
Western Blot (WB)
(Western blot analysis using PDK2 mouse mAb against Jurkat (1), C6 (2), Cos7 (3), K562 (4), A431 (5) cell lysate.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).)
UniPort Summary Function: Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
Protein type: ATYPICAL group; EC 2.7.11.2; Kinase, protein; Mitochondrial; PDHK family; PDHK subfamily; Protein kinase, atypical
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: cytoplasm; mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex; mitochondrion; nucleoplasm
Molecular Function: ATP binding; protein homodimerization activity; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity
Biological Process: cellular response to nutrient; glucose homeostasis; insulin receptor signaling pathway; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; regulation of pH
Research Articles on PDK2
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Product Notes
The PDK2 pdk2 (Catalog #AAA476767) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDK2 Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PDK2 pdk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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