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Western Blot (WB) (Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 kDa)

PDCD1 recombinant antibody

PDCD1 Antibody

Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity-chromatography
Synonyms
PDCD1; Monoclonal Recombinant Antibody; PDCD1 Antibody; Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279); PD1; PDCD1 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
8E9
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for PDCD1 recombinant antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:50-1:200
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human PD1
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 kDa)

Western Blot (WB) (Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PD1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 kDa)

Immunohistochemistry (IHC)

(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Related Product Information for PDCD1 recombinant antibody
Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Product Categories/Family for PDCD1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
31,647 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Research Articles on PDCD1

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Product Notes

The PDCD1 pdcd1 (Catalog #AAA7136580) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDCD1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PDCD1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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