Human PD1/PDCD1/CD279 Monoclonal Antibody | anti-PDCD1 antibody

PD1/PDCD1/CD279 Neutralizing Antibody

Cat. Num. AAA8102539

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human; Predicted to React with: KD of the antibody is <1.43E-10M for PD1 as measured by OCTET RED System.
• Applications: Neutralization Assay
• Purity: Protein A
• Synonyms: PD1/PDCD1/CD279; Monoclonal Antibody; PD1/PDCD1/CD279 Neutralizing Antibody; programmed cell death 1; Anti-CD279 Antibody; Anti-hPD-1 Antibody; Anti-hPD-l Antibody; Anti-hSLE1 Antibody; Anti-PD-1 Antibody; Anti-PD1 Antibody; Anti-SLEB2 Antibody; anti-PDCD1 antibody

• Host: Human
• Reactivity: Human; Predicted to React with: KD of the antibody is <1.43E-10M for PD1 as measured by OCTET RED System.
• Clonality: Monoclonal
• Isotype: Human IgG4
• Clone Number: HN94
• Specificity: Human PD-1.; No cross-reactivity in ELISA with Mouse PD1 Protein
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0

• Applicable Applications for anti-PDCD1 antibody: Blocking (BL), Neutralization
• Application Notes: Block: In a functional ELISA which immobilized recombinant Human PD1/Fc Chimera at 1ug/mL (100uL/well) in the plate, the Human PD1 Monoclonal Antibody can block the binding of 1ug/mL of biotinylated recombinant Human PD-L1 to human PD1, the EC50 is 0.5-1ug/mL.; Neutralization: The neutralization activity of PD1 Neutralizing Antibody is Measured by its ability to neutralize PD1_PD-L1 pathway mediated inhibition of IFN-gamma secretion in the CD4+ T cells.
• Immunogen: Recombinant Human PD1/PDCD1/CD279 Protein
• Endotoxin: <0.5EU/mg
• Conjugation: Unconjugated
• Preparation: This antibody was a humanized antibody obtained from a mouse immunized with the recombinant Human PD1/PDCD1 Protein and was produced using recombinant antibody technology.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Testing Data

(Effect of Human PD1 HN94 antibody on IFN-gamma production in the Mixed Lymphocyte Reaction (MLR). Purified CD4+ T cells were cultured with allogeneic dendritic cells, as prepared by culturing monocytes in GM-CSF and IL-4 medium for six days, in the medium containing serial concentrations of human PD1-HN94-IgG4. Five days later, the IFN-gamma production in the medium was evaluated (A), and the cell proliferation was measured by WST-8.assay (B).)

Testing Data

(Effect of Human PD1 HN94 antibody on IL-2 production in the Mixed Lymphocyte Reaction (MLR). Purified CD4+ T cells were cultured with allogeneic dendritic cells, as prepared by culturing monocytes in GM-CSF and IL-4 medium for six days, in the medium containing serial concentrations of human PD1-HN94-IgG4. Five days later, the IL-2 production in the medium was evaluated.)

Testing Data

(Safety informationNon specific binding in human tissuesNo non-specific or specific binding were detected on 12 human formalin-fixed, paraffin-embedded tissue sections using sensitive HPR detection system.)

Related Product Information for anti-PDCD1 antibody

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Product Categories/Family for anti-PDCD1 antibody

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
• Protein Family: Programmed cell death protein

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]