Rabbit anti-Human PD-L1/CD274 Monoclonal Antibody | anti-PD-L1/CD274 antibody

PD-L1/CD274

Cat. Num. AAA370318

• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: PD-L1/CD274; Monoclonal Antibody; anti-PD-L1/CD274 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: RBT-PDL1
• Form/Format: PD-L1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Applicable Applications for anti-PD-L1/CD274 antibody: Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Specimen Preparation:; Paraffin sections:; The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.; ; Frozen sections and cell preparations:; The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Localization: Membranous
• Control: Thymus, Tonsil, Placenta, Lymphoblastic Lymphoma, Hodgkin's Lymphoma Tissue
• Precautions: 1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 3. Dispose of unused solution with copious amount of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information refer to Safety Data Sheet for this product.
• Intended Use: For Analyte Specific Reagent.; ; This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
• Immunogen: Synthetic peptide corresponding to N-terminus residues of the human PD-L1 protein.
• Preparation and Storage: Store at 2 to 8 degree C.

Dilution Information

Immunohistochemistry (IHC)

Quality Control Procedure

(This Antibody has been quality control tested by immunohistochemistry as follows:Quality Control Procedure:)

Tissue Distribution

Related Product Information for anti-PD-L1/CD274 antibody

Programmed death-ligand 1 (PD-L1) also known as CD274 or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other diseases.The upregulation of PD-L1 may allow cancers to evade the host immune system. An analysis of tumor specimens from patients with renal cell carcinoma found that high tumor expression of PD-L1 was associated with increased tumor aggressiveness and a 4.5-fold increased risk of death. Ovarian cancer patients with higher expression of PD-L1 had a significantly poorer prognosis than those with lower expression. PD-L1 expression correlated inversely with intraepithelial CD8+ T-lymphocyte count, suggesting that PD-L1 on tumor cells may suppress antitumor CD8+ T cells. The PD-1/PD-L1 interaction is implicated in autoimmunity from several lines of evidence. In humans, PD-L1 was found to have altered expression in pediatric patients with Systemic lupus erythematosus. Studying isolated PBMC from healthy children, immature myeloid dendritic cells and monocytes expressed little PD-L1 at initial isolation, but spontaneously up-regulated PD-L1 by 24 hours. In contrast, both mDC and monocytes from patients with active SLE failed to upregulate PD-L1 over a 5 day time course, expressing this protein only during disease remissions.