Mouse PD-L1 Monoclonal Antibody | anti-PD-L1 antibody

mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal)

Cat. Num. AAA590085

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human. Others not tested.
• Applications: ELISA
• Purity: Protein G Affinity Purified
• Synonyms: PD-L1; Monoclonal Antibody; mAb Anti Human PD-L1 clone 12B4; Tracer (Biotin) (Monoclonal); mAb anti-Human PD-L1; Mouse monoclonal antibody (mAb) to human programmed death-ligand 1 (PD-L1); mouse mAb to B7H1; mouse mAb to human CD274; Tracer (Biotin Labeled); Biotin conjugated Mouse monoclonal antibody (mAb) to human programmed death-ligand 1 (PD-L1); anti-PD-L1 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 12B4
• Specificity: Reactive with human programmed death-ligand 1 (PD-L1).
• Purity/Purification: Protein G Affinity Purified
• Form/Format: Lyophilized from a solution in 0.01M PBS, pH 7.2
• Sequence Length: 245

• Applicable Applications for anti-PD-L1 antibody: ELISA (EIA)
• Application Notes: Sandwich ELISA: Biotin labelled anti-PD-L1 clone 12B4, when used in combination with clone 15G3 (Cat. NO.: MBS592438) coated plate, can detect human cell expressed PD-L1 in Sandwich ELISA application; The antibody reacts with recombinant PD-L1 in indirect ELISA.
• Immunogen: Human cell expressed recombinant PDL-1 (Phe19-Arg238) with poly-histidine tag at C-terminus.
• Light Chain: Kappa
• Fusion Myeloma: Sp2/0-Ag14
• Target Protein: Human programmed death-ligand 1 (PD-L1), also known as B7H1 or CD274.
• Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
• Note: Tracer antibody in match antibody pair, biotin conjugated antibody; Detection antibody in matched antibody pair, biotin conjugated.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freeze/thaw cycles after reconstitution.

Related Product Information for anti-PD-L1 antibody

PD-L1, a 40KD transmembrane protein, is a ligand of immune checkpoint protein Programmed Death-1 (PD-1). During T-cell receptor signalling, the binding of PD-L1 to PD-1 transmits an inhibitory signal to activated T-cells to decrease their proliferation. PD-L1 is also believed to play a role in the generation and regulation of Treg cells, and in the enhancement of Treg cell function. PD-L1/PD-1 pathway is important to immune suppression, such as self-tolerance, suppression of immune-rejection during pregnancy and allograft, and immune evasion by cancers. Upregulation of PD-L1 has been found to be associated with many cancer types. For example, increased PD-L1 expression has been shown to be related to tumor aggressiveness and higher mortality in patients with renal cell carcinoma, and related to significantly poorer prognosis and lower intraepithelial CD8+ T-lymphocyte count in patience with ovarian cancer. Antibodies that block the PD-L1 /PD-1 pathway have been extensively studied in clinical trials, and some have been approved as immunotherapy for cancer.

Product Categories/Family for anti-PD-L1 antibody

Immunosuppression; Cancer Immunotherapy

NCBI and Uniprot Product Information

• NCBI GI #: 930425329
• NCBI GeneID: 29126
• NCBI Accession #: NP_001300958.1
• NCBI GenBank Nucleotide #: NM_001314029.1
• Molecular Weight: 20,246 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform c
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: PD-L12 PublicationsManual assertion based on opinion iniRef.10; PDCD1 ligand 1; Programmed death ligand 1; B7-H11 PublicationManual assertion based on opinion iniRef.1

NCBI Description

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).