Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface flow analysis of mPD-1 in Con A treated Mouse Splenocytes using 0.5 ug/10^6 cells of mPD-1 antibody (Clone: ABM5F42). Green represents isotype control; red represents anti-mPD-1 antibody. Goat anti-rat PE conjugate was used as secondary antibody.)
Rat anti-Mouse PD-1 Monoclonal Antibody | anti-PD-1 antibody
Monoclonal Antibody to mPD-1 (Clone: ABM5F42)
Gene Names
Pdcd1; PD-1; Pdc1; Ly101
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
PD-1; Monoclonal Antibody; Monoclonal Antibody to mPD-1 (Clone: ABM5F42); Programmed cell death protein 1; Protein PD-1; CD279; anti-PD-1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
ABM5F42
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
288
Applicable Applications for anti-PD-1 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: 0.5-1 ug/10^6
Immunogen Information
partial length recombinant protein (a.a 07-211) of mPD-1 was used as the immunogen for this antibody.
Antigen
mPD-1
Conjugate
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface flow analysis of mPD-1 in Con A treated Mouse Splenocytes using 0.5 ug/10^6 cells of mPD-1 antibody (Clone: ABM5F42). Green represents isotype control; red represents anti-mPD-1 antibody. Goat anti-rat PE conjugate was used as secondary antibody.)
Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface flow analysis of mPD-1 in Con A treated Mouse Splenocytes using 0.5 ug/10^6 cells of mPD-1 antibody (Clone: ABM5F42). Green represents isotype control; red represents anti-mPD-1 antibody. Goat anti-rat PE conjugate was used as secondary antibody.)
Related Product Information for anti-PD-1 antibody
PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Product Categories/Family for anti-PD-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
31,842 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
Pdcd1
NCBI Official Synonym Symbols
PD-1; Pdc1; Ly101
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
Pdcd1
UniProt Synonym Gene Names
Pd1; Protein PD-1; mPD-1
Uniprot Description
PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.
Protein type: Apoptosis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1|1 D
Cellular Component: external side of plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: negative regulation of apoptosis; negative regulation of tolerance induction; positive regulation of apoptosis
Research Articles on PD-1
Similar Products
Product Notes
The PD-1 pdcd1 (Catalog #AAA668434) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to mPD-1 (Clone: ABM5F42) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PD-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the PD-1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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