Mouse anti-Human PBEF/Visfatin Monoclonal Antibody | anti-PBEF antibody

PBEF/Visfatin

Cat. Num. AAA201199

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: By protein-A affinity chromatography
• Synonyms: PBEF/Visfatin; Monoclonal Antibody; Nicotinamide phosphoribosyltransferase precursor; Pre-B-cell colony-enhancing factor 1; NAMPT; MGC117256; PBEF; PBEF1; VF; Visfatin; pre-B-cell colony enhancing factor 1; anti-PBEF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 4D5
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 491

• Applicable Applications for anti-PBEF antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity.
• Antigen Species: Human
• Immunogen: Recombinant human PBEF/Visfatin (1-491aa) purified from E. coli
• Conjugation: Unconjugated
• Note: This product was produced from tissue culture supernatant.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(ICC/IF analysis of PBEF/Visfatin in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Western Blot (WB)

(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PBEF/Visfatin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SW480 cell lysate )

Related Product Information for anti-PBEF antibody

Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Product Categories/Family for anti-PBEF antibody

Cardiovascular; Immunology; Metabolism; Neuroscience; Signal Transduction

References

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31; Hug C, et al., (2005) Science. 307:366-367

NCBI and Uniprot Product Information

• NCBI GI #: 5031977
• NCBI GeneID: 10135
• NCBI Accession #: NP_005737
• NCBI GenBank Nucleotide #: NP_005737.1
• UniProt Accession #: P43490
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
• UniProt Entry Name: NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: Cell cycle regulation; Transferase; EC 2.4.2.12; Cytokine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: extracellular space; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; cytokine activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; drug binding; nicotinamide phosphoribosyltransferase activity

Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process