Mouse Parvalbumin Monoclonal Antibody | anti-PVALB antibody
Parvalbumin
Gene Names
PVALB; D22S749; MGC116759; PVALB
Reactivity
Bovine, Canine, Feline, Frog, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
AscitesAscites
Synonyms
Parvalbumin; Monoclonal Antibody; Anti -Parvalbumin; anti-PVALB antibody
Host
Mouse
Reactivity
Bovine, Canine, Feline, Frog, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H43
Specificity
Recognizes frog Parvalbumin. Crossreactivity with respect to the isoforms (PARV-alpha and beta) has not been studied. No significant crossreactivity is seen with other CABPs or EF-hand containing proteins. Species Crossreactivity: bovine, human, mouse, rat, canine, feline and fish.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Applicable Applications for anti-PVALB antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent.
Western Blot: 1:2000-5000 (ECL). MW: ~12kD.
Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain.
Dilution: ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent.
Western Blot: 1:2000-5000 (ECL). MW: ~12kD.
Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain.
Immunogen
Purified frog muscle Parvalbumin
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-PVALB antibody
Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors.
Product Categories/Family for anti-PVALB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
12,059 Da
NCBI Official Full Name
parvalbumin
NCBI Official Synonym Full Names
parvalbumin
NCBI Official Symbol
PVALB
NCBI Official Synonym Symbols
D22S749; MGC116759; PVALB
NCBI Protein Information
parvalbumin alpha; OTTHUMP00000197806; OTTHUMP00000197807; OTTHUMP00000197808
UniProt Protein Name
Parvalbumin alpha
Protein Family
UniProt Gene Name
PVALB
UniProt Entry Name
PRVA_HUMAN
NCBI Description
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. [provided by RefSeq]
Uniprot Description
PVA: In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Belongs to the parvalbumin family.
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: protein complex; cell soma; axon; cytoplasm; nucleus
Molecular Function: protein homodimerization activity; protein heterodimerization activity; calcium ion binding
Biological Process: cytosolic calcium ion homeostasis
Research Articles on PVALB
1. sequencing of PVALB was performed in 132 cases of Gitelman's syndrome in whom only one or no (N = 79) mutant SLC12A3 allele was found
2. The results of this study indicate a significant reduction in the number of PV interneurons within layer 2 of the multiple sclerosis primary motor cortex
3. There is no correlation between total neuronal loss and PV-ir neuronal loss in any of the hippocampal fields in epileptic patient.
4. Slow relaxation caused by alpha-Tm mutants can be corrected by modifying calcium handling with parvalbumin.
5. demonstration of a reduced number of parvalbumin-immunoreactive projection neurons in the mammillary bodies in schizophrenia
6. In dopaminergic cells of the substantia nigra in Parkinson disease an increased parvalbumin content is detected reflecting a natural protective mechanism against putative increase of intracellular calcium caused by excitotoxic injury and oxidative stress.
7. In subjects with schizophrenia a reduction in parvalbumin [PV] and GAD67 mRNA expression in prefrontal cortex neurons was noted
2. The results of this study indicate a significant reduction in the number of PV interneurons within layer 2 of the multiple sclerosis primary motor cortex
3. There is no correlation between total neuronal loss and PV-ir neuronal loss in any of the hippocampal fields in epileptic patient.
4. Slow relaxation caused by alpha-Tm mutants can be corrected by modifying calcium handling with parvalbumin.
5. demonstration of a reduced number of parvalbumin-immunoreactive projection neurons in the mammillary bodies in schizophrenia
6. In dopaminergic cells of the substantia nigra in Parkinson disease an increased parvalbumin content is detected reflecting a natural protective mechanism against putative increase of intracellular calcium caused by excitotoxic injury and oxidative stress.
7. In subjects with schizophrenia a reduction in parvalbumin [PV] and GAD67 mRNA expression in prefrontal cortex neurons was noted
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Product Notes
The PVALB pvalb (Catalog #AAA603624) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Parvalbumin reacts with Bovine, Canine, Feline, Frog, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Parvalbumin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Dilution: ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent. Western Blot: 1:2000-5000 (ECL). MW: ~12kD. Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain. Researchers should empirically determine the suitability of the PVALB pvalb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parvalbumin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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