Western Blot (WB)
(Western blot analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points. The blots were probed with anti-Parp (cleavage specific) at 1:250. The band corresponding to cleaved PARP is 89kDa and is induced after 1 to 4 hrs of staurosporine treatment.)
Mouse PARP Monoclonal Antibody | anti-PARP antibody
PARP (cleavage specific) Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Protein G chromatography
Synonyms
PARP; Monoclonal Antibody; PARP (cleavage specific) Antibody; PARP (cleavage specific); anti-PARP antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
194C1439
Specificity
The antibody detects an 89kDa* protein corresponding to the molecular mass of the cleaved fragment of PARP induced by staurosporine treatment of Jurkat cells. The antibody can be used to identify caspase-3 activity during apoptosis.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide.
Applicable Applications for anti-PARP antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 1:500
WB: 1:500
WB: 1:500
Immunogen
Clone 194C1439 was generated from a synthetic peptide corresponding to amino acids around the 214/215-cleavage site in human PARP. This sequence is well conserved in rat and mouse PARP, but the antibody has not been tested for these species reactivities.
Preparation and Storage
Store at 4 degree C. For long term storage, store at -20 degree C.
Western Blot (WB)
(Western blot analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points. The blots were probed with anti-Parp (cleavage specific) at 1:250. The band corresponding to cleaved PARP is 89kDa and is induced after 1 to 4 hrs of staurosporine treatment.)
Western Blot (WB)
(Western blot analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points. The blots were probed with anti-Parp (cleavage specific) at 1:250. The band corresponding to cleaved PARP is 89kDa and is induced after 1 to 4 hrs of staurosporine treatment.)
Related Product Information for anti-PARP antibody
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kDa fragment containing the DNA binding domain and an 89kDa fragment containing the catalytic and automodification domains. The 24kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
References
Smulson, M.E. et al. (1998) Cancer Res. 58(16):3495.
Tewari, M. et al. (1995) Cell. 81:801.
Kaufmann, S.H. et al. (1993) Cancer Res. 53:3976.
Tewari, M. et al. (1995) Cell. 81:801.
Kaufmann, S.H. et al. (1993) Cancer Res. 53:3976.
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Product Notes
The PARP (Catalog #AAA474522) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PARP (cleavage specific) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PARP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). FC/FACS: 1:500 WB: 1:500. Researchers should empirically determine the suitability of the PARP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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