Immunohistochemistry (IHC)
(immunohistochemical staining of cleaved PARP in formalin-fixed, paraffin embedded human tonsillar tissue using MBS612865. Antigen retrieval done using citrate buffer.)
Rabbit PARP Cleavage Site, p85 Asp214/Gly215 Polyclonal Antibody
PARP Cleavage Site, p85 Asp214/Gly215 (Poly ADP-Ribose Polymerase)
Reactivity
Bovine, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PARP Cleavage Site; p85 Asp214/Gly215; Polyclonal Antibody; p85 Asp214/Gly215 (Poly ADP-Ribose Polymerase); Anti -PARP Cleavage Site; anti-PARP Cleavage Site; p85 Asp214/Gly215 antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Specifically recognizes the 85kD fragment of cleaved PARP and can be used as marker for detecting apoptotic cells.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, 1mg/ml BSA (IgG and protease free), 0.05% sodium azide.
Applicable Applications for anti-PARP Cleavage Site, p85 Asp214/Gly215 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic peptide corresponding to N-terminus of cleavage site (214/215) of human PARP.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(immunohistochemical staining of cleaved PARP in formalin-fixed, paraffin embedded human tonsillar tissue using MBS612865. Antigen retrieval done using citrate buffer.)
Immunohistochemistry (IHC)
(immunohistochemical staining of cleaved PARP in formalin-fixed, paraffin embedded human tonsillar tissue using MBS612865. Antigen retrieval done using citrate buffer.)
Related Product Information for anti-PARP Cleavage Site, p85 Asp214/Gly215 antibody
PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85kD and a 25kD fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP. The final product is generated by affinity chromatography using a peptide corresponding to the PARP cleavage site. Poly (ADP-Ribose) Polymerase (PARP) is a 116kD nuclear protein which is strongly activated by DNA strand breaks.
Product Categories/Family for anti-PARP Cleavage Site, p85 Asp214/Gly215 antibody
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Product Notes
The PARP Cleavage Site, p85 Asp214/Gly215 (Catalog #AAA612865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP Cleavage Site, p85 Asp214/Gly215 (Poly ADP-Ribose Polymerase) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP Cleavage Site, p85 Asp214/Gly215 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the PARP Cleavage Site, p85 Asp214/Gly215 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP Cleavage Site, p85 Asp214/Gly215, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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