Goat TPI1 / TPI Polyclonal Antibody | anti-TPI1 antibody

Goat Polyclonal to Human TPI1 / TPI

Cat. Num. AAA242130

• Gene Names: TPI1; TIM; TPI; TPID; HEL-S-49
• Reactivity: Bat, Gibbon, Bovine, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat; Predicted Reactivity: Pig, Chicken, Xenopus, Drosophila (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TPI1 / TPI; Polyclonal Antibody; Goat Polyclonal to Human TPI1 / TPI; Anti-TPI1 / TPI Antibody (C-Terminus) IHC-plus; TPI1; Triose-phosphate isomerase; Triosephosphate isomerase; TPI; Triosephosphate isomerase 1; TIM; Human TPI1; anti-TPI1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat; Predicted Reactivity: Pig, Chicken, Xenopus, Drosophila (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TPI1. This antibody is expected to recognise both reported isoforms.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-TPI1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (5 ug/ml), WB (0.01 - 0.03 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-LKPEFVDIINAKQ from the C-terminus of human TPI1 (NP_000356.1; NP_001152759.1).
• Immunogen Type: Synthetic peptide
• Immunogen: TPI1 / TPI antibody was raised against synthetic peptide C-LKPEFVDIINAKQ from the C-terminus of human TPI1 (NP_000356.1; NP_001152759.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Salmon, Pike (100%); Pig, Chicken, Xenopus, Smelt, Pufferfish, Drosophila (92%); Catfish, Stickleback, Zebrafish, Neurospora, Beetle, Sordaria, Tick, Chaetomium, Thielavia (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TPI1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody staining (0.003 ug/ml) of human liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507645
• NCBI GeneID: 7167
• NCBI Accession #: NP_000356.1
• NCBI GenBank Nucleotide #: NM_000365.5
• UniProt Accession #: P60174; P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9
• Molecular Weight: 17,958 Da
• NCBI Official Full Name: triosephosphate isomerase isoform 1
• NCBI Official Synonym Full Names: triosephosphate isomerase 1
• NCBI Official Symbol: TPI1
• NCBI Official Synonym Symbols: TIM; TPI; TPID; HEL-S-49
• NCBI Protein Information: triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49
• UniProt Protein Name: Triosephosphate isomerase
• UniProt Gene Name: TPI1
• UniProt Synonym Gene Names: TPI; TIM
• UniProt Entry Name: TPIS_HUMAN

NCBI Description

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular space; nucleus; cytosol

Molecular Function: protein binding; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Triosephosphate Isomerase Deficiency