Mouse anti-Human, Mouse p57Kip2 Monoclonal Antibody | anti-CDKN1C antibody

p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody

Cat. Num. AAA438749

• Gene Names: CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: p57Kip2; Monoclonal Antibody; p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody; Beckwith Wiedemann syndrome (WBS); BWCR; Cyclin dependent kinase inhibitor 1C (CDKN1C); Cyclin dependent kinase inhibitor p57; KIP2; p57; anti-CDKN1C antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: SPM308
• Specificity: Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 316

• Applicable Applications for anti-CDKN1C antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclear
• Immunogen: Recombinant human p57Kip2 protein
• Hu-Chromosome Location: 11p15.5
• Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p57 Monoclonal Antibody (SPM308).)

References

Lee, M.-H., et al. 1995. Cloning of p57, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9: 639-649.

NCBI and Uniprot Product Information

• NCBI GI #: 4557441
• NCBI GeneID: 1028
• NCBI Accession #: NP_000067.1
• NCBI GenBank Nucleotide #: NM_000076.2
• UniProt Accession #: P49918
• Molecular Weight: 57kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1C isoform a
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1C
• NCBI Official Symbol: CDKN1C
• NCBI Official Synonym Symbols: BWS; WBS; p57; BWCR; KIP2; p57Kip2
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1C
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1C
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN1C
• UniProt Synonym Gene Names: KIP2
• UniProt Entry Name: CDN1C_HUMAN

NCBI Description

This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

p57Kip2: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life. Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. Belongs to the CDI family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Tumor suppressor; Inhibitor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: negative regulation of epithelial cell proliferation; negative regulation of kinase activity; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway

Disease: Beckwith-wiedemann Syndrome; Intrauterine Growth Retardation, Metaphyseal Dysplasia, Adrenal Hypoplasia Congenita, And Genital Anomalies