Human 28S Ribosomal Protein S31, Mitochondrial (MRPS31) ELISA Kit | MRPS31 elisa kit

Human 28S Ribosomal Protein S31, Mitochondrial (MRPS31) ELISA Kit

Cat. Num. AAA9715191

• Gene Names: MRPS31; S31mt; IMOGN38; MRP-S31
• Reactivity: Human
• Synonyms: 28S Ribosomal Protein S31; Mitochondrial (MRPS31); Human 28S Ribosomal Protein S31; Mitochondrial (MRPS31) ELISA Kit; MRPS31; IMOGN38; MRP-S31; S31mt; imogen 38; MRPS31 elisa kit

• Reactivity: Human
• Specificity: Human 28S ribosomal protein S31, mitochondrial (MRPS31) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS31. No significant cross-reactivity or interference between Human MRPS31 and analogues was observed.
• Sequence Length: 395

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human 28S ribosomal protein S31, mitochondrial (MRPS31) Standard Curve.)

Related Product Information for MRPS31 elisa kit

Description: This Human 28S ribosomal protein S31, mitochondrial (MRPS31) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS31 in samples. An antibody specific for MRPS31 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MRPS31 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS31 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS31 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. MRPS31 encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to MRPS31 have been found on chromosomes 3 and 13.

NCBI and Uniprot Product Information

• NCBI GI #: 186928854
• NCBI GeneID: 10240
• NCBI Accession #: NP_005821.2
• NCBI GenBank Nucleotide #: NP_005821.2
• UniProt Accession #: Q92665
• NCBI Official Full Name: 28S ribosomal protein S31, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S31
• NCBI Official Symbol: MRPS31
• NCBI Official Synonym Symbols: S31mt; IMOGN38; MRP-S31
• NCBI Protein Information: 28S ribosomal protein S31, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S31, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS31
• UniProt Synonym Gene Names: IMOGN38; MRP-S31; S31mt
• UniProt Entry Name: RT31_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13. [provided by RefSeq, Jul 2008]