Mouse anti-Human p19skp1 Monoclonal Antibody

p19skp1 (S-phase Kinase-associated Protein)

Cat. Num. AAA600747

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: p19skp1; Monoclonal Antibody; p19skp1 (S-phase Kinase-associated Protein); Anti -p19skp1 (S-phase Kinase-associated Protein); anti-p19skp1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G222
• Specificity: Recognizes human SKP1 (p19) protein at 19kD.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-p19skp1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: ELISA: 0.1-1.0ug/mL; Western Blot: 1-3ug/ml; Immunoprecipitation: 5-10ug/ml
• Immunogen: Recombinant human SKP1 (p19) protein.
• Positive Control: HeLa cervical carcinoma, Jurkat leukemia T cell and Wi-38 lung broblast cell lysates.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-p19skp1 antibody

The SKP1 gene was first identified in yeast, where it is essential for the G1/S and G2/M cell cycle transitions.1 Its protein product, Skp1 (p19), interacts with cyclin F directly and cyclin A indirectly, and is thought to link these key regulatory proteins of the cell cycle to ubiquitin-mediated degradation by the proteolytic pathway. Skp1 is a subunit of a type of E3 ubiquitin ligase known as an SCF ligase, for its Skp1, Cul1, and F-box protein components. Within the SCF complex, Cul1 functions as the catalytic core which recruits the E2 ubiquitin-conjugating enzyme, the F-box protein provides substrate specificity for the protein to be degraded,2 and Skp1 acts as an adapter protein linking the F-box protein to Cul1.3 De-regulation of SCF-dependent protein degradation has been implicated as a contributing factor in malignant transformation. The complete loss or increased proteolysis of cyclin E, b-catenin, and p27, all of which are degradation targets of specific SCF ligases, has been observed as a frequent event in cancer.3 The human SCF ligase containing Skp2 as its F-box protein (SCFSkp2) degrades p27, SCFTrcp targets b-catenin,4 and SCFFbw7/hCdc4/hAgo degrades cyclin E.

Product Categories/Family for anti-p19skp1 antibody

Antibodies; Abs to Cancer Markers