Mouse anti-Human, Rat p130Cas Monoclonal Antibody | anti-p130Cas antibody

p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein) (BSA & Azide Free) (FITC)

Cat. Num. AAA6266589

• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: p130Cas; Monoclonal Antibody; p130Cas (Cas for Crk-associated substrate; BCAR1; K-associated Substrate; Breast Cancer anti-estrogen Resistance 1 Protein) (BSA & Azide Free) (FITC); anti-p130Cas antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F309
• Specificity: Recognizes human p130Cas. Species Crossreactivity: rat.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-p130Cas antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant human p130cas protein. MW of Antigen: 130kD. Cellular Localization: Cytoplasmic.
• Conjugate: FITC
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded rat breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)

Testing Data

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Testing Data

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Product Categories/Family for anti-p130Cas antibody

Antibodies; Apoptosis

References

1. Sakai, R., et al., EMBO J. 13(16): 3748-3756 (1994). 2. Nojima, Y., et al., J. Biol. Chem. 270: 15398-15402 (1995). 3. Guan, J.L.,: Focal adhesion kinase in integrin signaling. Matrix Biol. 16(4): 195-200 (1997, Oct). 2. Nakamoto, T., Sakai, R., Honda, H., Ogawa, S., Ueno, H., Suzuki, T., Aizawa, S., Yazaki, Y., Hirai, H.,: Requirements for localization of p130cas to focal adhesions. Mol. Cell. Biol. 17(7): 3884-3897 (1997, Jul). 4. Sorokin, A., Reed, E.,: Insulin stimulates the tyrosine dephosphorylation of docking protein p130cas (Crk-associated substrate), promoting the switch of the adaptor protein crk from p130cas to newly phosphorylated insulin receptor substrate-1. Biochem. J. 334 (Pt 3): 595-600 (1998, Sep 15). 5. Ojaniemi, M., Vuori, K.,: Epidermal growth factor modulates tyrosine phosphorylation of p130Cas. Involvement of phosphatidylinositol 3'-kinase and actin cytoskeleton. J. Biol. Chem. 272(41): 25993-25998 (1997). 5. Honda, H., Oda, H., Nakamoto, T., Honda, Z., Sakai, R., Suzuki, T., Saito, T., Nakamura, K., Nakao, K., Ishikawa, T., Katsuki, M., Yazaki, Y., Hirai, H.,: Cardiovascular anomaly, impaired actin bundling and resistance to Src-induced transformation in mice lacking p130Cas. Nat. Genet. 19(4): 361-365 (1998). 6. Etienne, S., Adamson, P., Greenwood, J., Strosberg, A.D., Cazaubon, S., Couraud, P.O.,: ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells. J. Immunol. 161(10): 5755- 5761 (1998, Nov 15). 7. Sayeski, P.P., Ali, M.S., Harp, J.B., Marrero, M.B., Bernstein, K.E.,: Phosphorylation of p130Cas by angiotensin II is dependent on c-Src, intracellular Ca2+, and protein kinase C. Circ. Res. 82(12): 1279-1288 (1998, Jun 29). 8. Nakamura, I., Jimi, E., Duong, L.T., Sasaki, T., Takahashi, N., Rodan, G.A., Suda, T.,: Tyrosine phosphorylation of p130Cas is involved in actin organization in osteoclasts. J. Biol. Chem. 273(18): 11144-11149 (1998, May 1). 9. Polte, T.R., Hanks, S.K.,: Interaction between focal adhesion kinase and Crk-associated tyrosine kinase substrate p130Cas. PNAS USA 92(23): 10678-10682 (1995, Nov 7). 10. Klemke, R.L., Leng, J., Molander, R., Brooks, P.C., Vuori, K., Cheresh, D.A.,: CAS/Crk coupling serves as a "molecular switch" for induction of cell migration. J. Cell. Biol. 140(4): 961- 972 (1998, Feb 23).