Rat anti-Mouse OSCAR Monoclonal Antibody | anti-OSCAR antibody
OSCAR, Mouse, mAb 5B8
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
OSCAR; Monoclonal Antibody; Mouse; mAb 5B8; clone 5B8; anti-OSCAR antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-OSCAR antibody
Flow Ctometry (FC/FACS), Functional Studies (FS)
Application Notes
For flow cytometry and Immuno fluorescence, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-OSCAR antibody
The monoclonal antibody 5B8 reacts with mouse osteoclast-associated receptor (OSCAR), which is a 265 amino acid type 1 transmembrane protein belonging to the leukocyte receptor complex protein family that play critical roles in the regulation of both innate and adaptive immune responses. In mice, OSCAR is a RANKL-inducible gene which- is expressed during the later stages of pre-osteoclast maturation and is shown to be a potent FcRγ-associated costimulatory receptor in vitro. OSCAR promotes osteoclastogenesis in vivo, and OSCAR binding to its collagen ligand led to signaling that- is involved in functional differentiation of leukocytes. In recent studies human OSCAR has been shown to play multiple roles in the immune system including activation and maturation of immune cells, prevention of apoptosis and enhancement of pro-inflammatory circuits. The latter by- inducing intracellular calcium release resulting in sustained IL-8 and chemokine expression leading to chemotaxis of Th2 effector and regulatory T-cells. In a- murine model using calvarias bone explants, the synovial fluid of osteoarthritis patients resulted in increased OSCAR mRNA expression suggesting that OSCAR might contribute to in the degenerative process. Moreover, in a recent study- OSCAR was found to be- induced in monocytes of RA patients, facilitating their differentiation into osteoclasts and bone resorption. The monoclonal antibody, 5B8, can be used for analysis of mouse OSCAR in flow-cytometry as well as functional studies for osteoclastogenesis.
References
1. Koga, T et al; Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature 2004, 428: 758. 2. Hara, H et al; Cell type-specific regulation of ITAM-mediated NF-{kappa}B activation by the adaptors, CARMA1- and CARD9. J. Immunol 2008, 181: 918.
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Product Notes
The OSCAR (Catalog #AAA584680) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The OSCAR, Mouse, mAb 5B8 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OSCAR can be used in a range of immunoassay formats including, but not limited to, Flow Ctometry (FC/FACS), Functional Studies (FS). For flow cytometry and Immuno fluorescence, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Researchers should empirically determine the suitability of the OSCAR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSCAR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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