Mouse Nuclear Pore Complex, nup153 Monoclonal Antibody | anti-NUP153 antibody

Nuclear Pore Complex, nup153 (NPC)

Cat. Num. AAA600797

• Gene Names: NUP153; N153; HNUP153
• Reactivity: Human, Mouse, Rat, Rodent, Xenopus
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Nuclear Pore Complex; nup153; Monoclonal Antibody; nup153 (NPC); Anti -Nuclear Pore Complex; anti-NUP153 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Rodent, Xenopus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5F280
• Specificity: Recognizes the nuclear pore complex (NPC) protein nup153 (190kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid, 0.09% sodium azide.

• Applicable Applications for anti-NUP153 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry.; Dilution: Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde).; Western Blot: 1:30-1:50 (ECL method).
• Immunogen: Nuclear matrix protein fraction prepared from rat liver nuclei.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-NUP153 antibody

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family.

Product Categories/Family for anti-NUP153 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24430146
• NCBI GeneID: 9972
• NCBI Accession #: NP_005115.2
• NCBI GenBank Nucleotide #: NM_005124.2
• UniProt Accession #: P49790; Q5T9I7; Q4LE47; Q7Z743
• Molecular Weight: 153,938 Da
• NCBI Official Full Name: nuclear pore complex protein Nup153
• NCBI Official Synonym Full Names: nucleoporin 153kDa
• NCBI Official Symbol: NUP153
• NCBI Official Synonym Symbols: N153; HNUP153
• NCBI Protein Information: nuclear pore complex protein Nup153; OTTHUMP00000016070; nucleoporin Nup153; 153 kDa nucleoporin; nuclear pore complex protein hnup153
• UniProt Protein Name: Nuclear pore complex protein Nup153
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP153
• UniProt Entry Name: NU153_HUMAN

NCBI Description

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. [provided by RefSeq]

Uniprot Description

NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Protein type: Nucleoporin; Nuclear export

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nuclear pore; nuclear inclusion body

Molecular Function: identical protein binding; protein binding; DNA binding; structural constituent of nuclear pore; zinc ion binding; transporter activity; protein anchor; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; entry of virus into host cell; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; negative regulation of RNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport