Typical Testing Data/Standard Curve (for reference only)
Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit | Hsd3b1 elisa kit
Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit
Gene Names
Hsd3b1; D3Ertd383e; 3-beta-HSD I
Reactivity
Mouse
Synonyms
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Hsd3b1; Hsd3b; Hsd3b1 elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Sequence Length
373
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
78-5000 pg/mL
Intra-assay Precision
Intra-Assay CV: <=8.3%
Inter-assay Precision
Inter-Assay CV: <=10.2%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Hsd3b1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,062 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol
Hsd3b1
NCBI Official Synonym Symbols
D3Ertd383e; 3-beta-HSD I
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Protein Family
UniProt Gene Name
Hsd3b1
UniProt Synonym Gene Names
Hsd3b; 3-beta-HSD I
UniProt Entry Name
3BHS1_MOUSE
Uniprot Description
HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.
Protein type: EC 1.1.1.145; EC 5.3.3.1; Isomerase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Mitochondrial; Oxidoreductase
Cellular Component: mitochondrion
Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity
Biological Process: steroid biosynthetic process
Research Articles on Hsd3b1
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Product Notes
The Mouse Hsd3b1 hsd3b1 (Catalog #AAA2887667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887667 ELISA Kit recognizes Mouse Hsd3b1. It is sometimes possible for the material contained within the vial of "3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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