Immunofluorescence (IF)
(Immunofluorescence Analysis of PFA-fixed HeLa cells with Hu Nuclear Antigen Recombinant Rabbit Monoclonal Antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green). )
Rabbit Nuclear Antigen (HNA) Monoclonal Antibody | anti-HNA antibody
Human Nuclear Antigen (HNA) (Human Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone 235-1R]
Reactivity
Human and Non-human PrimatesDoes not react with Mouse, Rat and chicken.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunocytochemistry
Synonyms
Nuclear Antigen (HNA); Monoclonal Antibody; Human Nuclear Antigen (HNA) (Human Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone 235-1R]; Human Nuclear Antigen (HNA) (Human Cell Marker); HNA; HuNu; anti-HNA antibody
Host
Rabbit
Reactivity
Human and Non-human Primates
Does not react with Mouse, Rat and chicken.
Does not react with Mouse, Rat and chicken.
Clonality
Monoclonal
Isotype
Rabbit/IgG, kappa
Clone Number
235-1R
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-HNA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
IF: 1-2 g/500ug protein lysate),ICC: Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT
Immunohistology: Frozen): 1-2ug/ml for 30 minutes at RT
IF: 1-2ug/ml
IF: 1-2 g/500ug protein lysate),ICC: Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT
Immunohistology: Frozen): 1-2ug/ml for 30 minutes at RT
Antibody Type
Recombinant Antibody
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Cellular Localization
Nuclei
Positive Control
All human cells. Human tonsil.
Hu-chromosome Location
Not Known
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunofluorescence (IF)
(Immunofluorescence Analysis of PFA-fixed HeLa cells with Hu Nuclear Antigen Recombinant Rabbit Monoclonal Antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green). )
Immunofluorescence (IF)
(Immunofluorescence Analysis of PFA-fixed HeLa cells with Hu Nuclear Antigen Recombinant Rabbit Monoclonal Antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green). )
Related Product Information for anti-HNA antibody
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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Product Notes
The HNA (Catalog #AAA4382446) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Human Nuclear Antigen (HNA) (Human Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone 235-1R] reacts with Human and Non-human Primates Does not react with Mouse, Rat and chicken. and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Antigen (HNA) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml IF: 1-2 g/500ug protein lysate),ICC: Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT Immunohistology: Frozen): 1-2ug/ml for 30 minutes at RT. Researchers should empirically determine the suitability of the HNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Antigen (HNA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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