Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).)
Mouse NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody | anti-NSE gamma antibody
NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)
Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
NSE gamma (Neuron Specific Enolase; gamma); Monoclonal Antibody; gamma) (Neuroendocrine Marker); 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE; anti-NSE gamma antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
SPM347
Specificity
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
434
Applicable Applications for anti-NSE gamma antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.1-0.2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.1-0.2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
A synthetic peptide corresponding to aa416-433 of human NSE gamma
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12p13
Positive Control
HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).)
References
Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~50kDa
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival ().
Research Articles on NSE gamma
Similar Products
Product Notes
The NSE gamma eno2 (Catalog #AAA439933) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's NSE gamma (Neuron Specific Enolase, gamma) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IF: 1-2ug/ml WB: 0.5-1ug/ml IHC-F: 0.1-0.2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the NSE gamma eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSE gamma (Neuron Specific Enolase, gamma), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
