Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Neuroendocrine tumor stained with NSE gamma Mouse Monoclonal Antibody (NSE-P2).)
Mouse anti-Human NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody | anti-ENO2 antibody
NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)
Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
NSE gamma (Neuron Specific Enolase; gamma); Monoclonal Antibody; gamma) (Neuroendocrine Marker); 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE; anti-ENO2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
NSE-P2
Specificity
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
434
Applicable Applications for anti-ENO2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12p13
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Neuroendocrine tumor stained with NSE gamma Mouse Monoclonal Antibody (NSE-P2).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Neuroendocrine tumor stained with NSE gamma Mouse Monoclonal Antibody (NSE-P2).)
SDS-Page
(SDS-PAGE Analysis Purified NSE gamma Mouse Monoclonal Antibody (NSE-P2). Confirmation of Purity and Integrity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified NSE gamma Mouse Monoclonal Antibody (NSE-P2). Confirmation of Purity and Integrity of Antibody.)
References
Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~50kDa
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; photoreceptor inner segment; phosphopyruvate hydratase complex; plasma membrane; perikaryon; cytosol
Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity
Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Research Articles on ENO2
Similar Products
Product Notes
The ENO2 eno2 (Catalog #AAA4380858) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NSE gamma (Neuron Specific Enolase, gamma) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. FC/FACS: 0.5-1ug/million cells IF: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSE gamma (Neuron Specific Enolase, gamma), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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