Human Natural resistance-associated macrophage protein 2 ELISA Kit | SLC11A2 elisa kit

Human Natural resistance-associated macrophage protein 2 ELISA Kit

Cat. Num. AAA765330

• Gene Names: SLC11A2; DCT1; DMT1; NRAMP2
• Reactivity: Human
• Synonyms: Natural resistance-associated macrophage protein 2; Human Natural resistance-associated macrophage protein 2 ELISA Kit; SLC11A2/DCT1/DMT1/DMT-1/NRAMP2/Solute carrier family 11 member 2/Divalent cation transporter 1/Divalent metal transporter 1; SLC11A2 elisa kit

• Reactivity: Human
• Sequence Length: 568

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SLC11A2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SLC11A2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SLC11A2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SLC11A2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SLC11A2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 295293169
• NCBI GeneID: 4891
• NCBI Accession #: NP_001167597.1
• NCBI GenBank Nucleotide #: NM_001174126.1
• Molecular Weight: 61,048 Da
• NCBI Official Full Name: natural resistance-associated macrophage protein 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 11 member 2
• NCBI Official Symbol: SLC11A2
• NCBI Official Synonym Symbols: DCT1; DMT1; NRAMP2
• NCBI Protein Information: natural resistance-associated macrophage protein 2
• UniProt Protein Name: Natural resistance-associated macrophage protein 2
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: SLC11A2
• UniProt Synonym Gene Names: DCT1; DMT1; NRAMP2; NRAMP 2; DMT-1
• UniProt Entry Name: NRAM2_HUMAN

NCBI Description

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]

Uniprot Description

SLC11A2: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA). The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. Belongs to the NRAMP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: apical part of cell; apical plasma membrane; brush border membrane; cell surface; cytoplasm; cytoplasmic vesicle; early endosome; integral to plasma membrane; late endosome; late endosome membrane; lysosomal membrane; lysosome; membrane; mitochondrial outer membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome; retromer complex; trans-Golgi network; vacuole

Molecular Function: cadmium ion binding; cadmium ion transmembrane transporter activity; calcium ion transmembrane transporter activity; cobalt ion transmembrane transporter activity; copper ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; ferrous iron uptake transmembrane transporter activity; hydrogen ion transmembrane transporter activity; inorganic cation transmembrane transporter activity; lead ion transmembrane transporter activity; manganese ion transmembrane transporter activity; nickel ion transmembrane transporter activity; protein binding; solute:hydrogen symporter activity; transferrin transmembrane transporter activity; transition metal ion transmembrane transporter activity; vanadium ion transmembrane transporter activity; zinc ion transmembrane transporter activity

Biological Process: caspase activation; cellular iron ion homeostasis; cobalt ion transport; copper ion transport; dendrite morphogenesis; detection of oxygen; erythrocyte development; ferrous iron transport; heme biosynthetic process; lead ion transport; learning and/or memory; manganese ion transport; nickel ion transport; response to hypoxia; response to iron ion; transferrin transport; transmembrane transport; vanadium ion transport

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 1