Mouse anti-Human NR3C2 Monoclonal Antibody | anti-NR3C2 antibody

NR3C2 (Mineralocorticoid Receptor, MR, Nuclear Receptor Subfamily 3 Group C Member 2, MCR, MLR) (AP)

Cat. Num. AAA6132651

• Gene Names: NR3C2; MR; MCR; MLR; NR3C2VIT
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR3C2; Monoclonal Antibody; NR3C2 (Mineralocorticoid Receptor; MR; Nuclear Receptor Subfamily 3 Group C Member 2; MCR; MLR) (AP); anti-NR3C2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2B5
• Specificity: Recognizes human NR3C2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NR3C2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-110 from human NR3C2 (NP_000892) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMD
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.84kD).)

Related Product Information for anti-NR3C2 antibody

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Product Categories/Family for anti-NR3C2 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 158508572
• NCBI GeneID: 4306
• NCBI Accession #: NP_000892
• NCBI GenBank Nucleotide #: NM_000901
• UniProt Accession #: P08235
• Molecular Weight: 107kDa
• NCBI Official Full Name: mineralocorticoid receptor isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3 group C member 2
• NCBI Official Symbol: NR3C2
• NCBI Official Synonym Symbols: MR; MCR; MLR; NR3C2VIT
• NCBI Protein Information: mineralocorticoid receptor
• UniProt Protein Name: Mineralocorticoid receptor
• Protein Family: Mineralocorticoid receptor
• UniProt Gene Name: NR3C2
• UniProt Synonym Gene Names: MCR; MLR; MR
• UniProt Entry Name: MCR_HUMAN

NCBI Description

This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

MCR: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1). PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported. Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP). Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. Belongs to the nuclear hormone receptor family. NR3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 4q31.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; receptor complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; signal transduction

Disease: Hypertension, Early-onset, Autosomal Dominant, With Severe Exacerbation In Pregnancy; Pseudohypoaldosteronism, Type I, Autosomal Dominant