Testing Data
(Rat brain microsome extract probed with N5380-05 (0.5ug/ml).)
Mouse anti-Rat NR1 Monoclonal Antibody | anti-NR1 antibody
NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1) (AP)
Gene Names
Grin1; NR1; GluN1; NMDAR1
Reactivity
Rat
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified by protein G affinity chromatography from ascites.
Synonyms
NR1; Monoclonal Antibody; CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1) (AP); anti-NR1 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
0.T.190
Specificity
Recognizes rat NR1 (Mr 130kD). An additional band (Mr 200kD) may be detected at higher concentrations. The epitope maps to a region (aa834-864) common to all NR1 isoforms and so should crossreact with all NR1 isoforms.
Purity/Purification
Purified by protein G affinity chromatography from ascites.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-NR1 antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
His tag fusion protein corresponding to aa834-938 of rat NR1. Peptide sequence homology: human and mouse, 100%
Conjugate
AP
Positive Control
Brain tissue
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Rat brain microsome extract probed with N5380-05 (0.5ug/ml).)
Testing Data
(Rat brain microsome extract probed with N5380-05 (0.5ug/ml).)
Product Categories/Family for anti-NR1 antibody
References
1. Hisatsune, C., et al., J. Biol. Chem. 272: 20805-20810, 1997. 2. Leonard, A.S. and J.W. Hell, J. Biol. Chem. 272: 12107-12115, 1997. 3. Raymond, L.A., et al., J. Physiol. Paris. 88: 181-192, 1994. 4. Tingley, W.G., et al., Nature 364: 70-73, 1993.5. Tingley, W.G., et al., J. Biol. Chem. 272: 5157-5166, 1997. 6. Molinuevo, J.L., et al. (2005) Am. J. Alzheimers Dis. Other Demen. 20: 77-85. 7. Popescu, G., and Auerbach, A. (2004). Neuroscientist. 210: 192-198.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,987 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 1 isoform 1b
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 1
NCBI Official Symbol
Grin1
NCBI Official Synonym Symbols
NR1; GluN1; NMDAR1
NCBI Protein Information
glutamate receptor ionotropic, NMDA 1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 1
Protein Family
UniProt Gene Name
Grin1
UniProt Synonym Gene Names
Nmdar1; GluN1; NMD-R1
NCBI Description
subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]
Uniprot Description
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
Research Articles on NR1
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Product Notes
The NR1 grin1 (Catalog #AAA6241541) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1) (AP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NR1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB). WB: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NR1 grin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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