Mouse NQO1 Monoclonal Antibody | anti-NQO1 antibody

MOUSE ANTI HUMAN NQO1

Cat. Num. AAA219386

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: NQO1; Monoclonal Antibody; MOUSE ANTI HUMAN NQO1; anti-NQO1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: A180
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 236

• Applicable Applications for anti-NQO1 antibody: Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 31kDa in HeLa and HepG2 cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Histology Positive Control Tissue: Human lung; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Hela lysate probed with Mouse anti Human NQO1 (MBS219416))

Testing Data

(Hela lysate probed with Mouse anti Human NQO1:HRP (MBS219472))

Related Product Information for anti-NQO1 antibody

Mouse anti human NQO1 antibody, clone A180 specifically recognises human NQO1 (NAD(P)H:quinone oxidoreductase 1), a 31kDa cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones. NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). Clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Sato, M. et al. 2010 and Fegerholm, R. et al. 2008). Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegal, D. et al. 1998). Clone A180 is reported as suitable for use in immunofluorescence studies.

NCBI and Uniprot Product Information

• NCBI GI #: 4505415
• NCBI GeneID: 1728
• NCBI Accession #: NP_000894.1
• NCBI GenBank Nucleotide #: NM_000903.2
• UniProt Accession #: P15559; Q86UK1; B2R5Y9; B4DNM7; B7ZAD1
• Molecular Weight: 26,365 Da
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H dehydrogenase, quinone 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; DT-diaphorase; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); diaphorase-4; dioxin-inducible 1; menadione reductase; phylloquinone reductase; quinone reductase 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1
• UniProt Entry Name: NQO1_HUMAN

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.6.5.2

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell soma; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity

Biological Process: response to ethanol; removal of superoxide radicals; positive regulation of neuron apoptosis; response to toxin; xenobiotic metabolic process; negative regulation of catalytic activity; regulation of amino acid metabolic process; nitric oxide biosynthetic process; response to nutrient; response to estradiol stimulus; synaptic transmission, cholinergic; aging