Rabbit anti-Human, Mouse NQO1 Polyclonal Antibody | anti-NQO1 antibody

NQO1 (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, QR1, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, DIA4, NMOR1) (AP)

Cat. Num. AAA6387359

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NQO1; Polyclonal Antibody; NQO1 (NAD(P)H Dehydrogenase [Quinone] 1; Azoreductase; DT-diaphorase; DTD; QR1; Menadione Reductase; NAD(P)H:quinone Oxidoreductase 1; Phylloquinone Reductase; Quinone Reductase 1; DIA4; NMOR1) (AP); anti-NQO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NQO1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NQO1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human NQO1, aa1-274 (NP_000894.1).
• Immunogen Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NQO1 rabbit polyclonal antibody. Western Blot analysis of NQO1 expression in human stomach.)

Western Blot (WB)

(NQO1 rabbit polyclonal antibody. Western Blot analysis of NQO1 expression in mouse brain.)

Western Blot (WB)

(NQO1 rabbit polyclonal antibody. Western Blot analysis of NQO1 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 polyclonal antibody. Lane 1: NQO1 transfected lysate (30.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-NQO1 antibody

NQO1 is a 274aa protein belonging to the NAD(P)H dehydrogenase (quinone) family. Its enzymatic activity prevents reduction of quinones that results in the production of radical species and is involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It plays a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome and regulates p53 proteasomal degradation in cancer. Defects in NQO1 lead to tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, susceptibility to various forms of cancer and Alzheimer's disease (AD). It is widely expressed.

Product Categories/Family for anti-NQO1 antibody

Antibodies; Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 4505415
• NCBI GeneID: 1728
• NCBI Accession #: NP_000894.1
• NCBI GenBank Nucleotide #: NM_000903.2
• UniProt Accession #: P15559; Q86UK1; B2R5Y9; B4DNM7; B7ZAD1
• Molecular Weight: 26,365 Da
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H dehydrogenase, quinone 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; DT-diaphorase; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; azoreductase; diaphora
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1
• UniProt Entry Name: NQO1_HUMAN

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.6.5.2

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell soma; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity

Biological Process: response to ethanol; removal of superoxide radicals; response to toxin; xenobiotic metabolic process; positive regulation of neuron apoptosis; negative regulation of catalytic activity; regulation of amino acid metabolic process; nitric oxide biosynthetic process; response to nutrient; response to estradiol stimulus; aging; synaptic transmission, cholinergic