Mouse anti-Human Notch1, Intracellular Domain Monoclonal Antibody | anti-NOTCH1 antibody

Notch1, Intracellular Domain (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

Cat. Num. AAA601902

• Gene Names: NOTCH1; hN1; TAN1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: Notch1; Intracellular Domain; Monoclonal Antibody; Intracellular Domain (Notch Homolog Protein 1; hN1; Translocation-associated Notch Protein TAN-1); Anti -Notch1; anti-NOTCH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8H191
• Specificity: Recognizes human Notch1 Intracellular Domain. Does not crossreact with recombinant human Notch2, Notch3 or Notch4.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-NOTCH1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry., Recommended Dilutions:, Western Blot: 1ug/ml detects Notch 1 in recombinant human Notch1 Fc Chimera., Immunocytochemistry: 8-25ug/ml for immersion fixed Saos-2 human osteosarcoma cells., Optimal dilutions to be determined by the researcher., Storage and Stability:, Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Immunogen: E. coli-derived recombinant human Notch1 (Gly2428-Lys2556, Accession #AAG33848)
• Preparation and Storage: -20 degree C

Related Product Information for anti-NOTCH1 antibody

Notch1 is a 300kD type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues. Notch can specify binary cell fates like promoting T- over B-cell development from a common precursor. > 50% of human T-lineage acute lymphoblastic leukemia (T-ALL) have activating mutations of Notch1. Human Notch1 is synthesized as a 2556aa precursor that contains an 18aa signal sequence, a 1718aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23aa transmembrane (TM) segment and a 785aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans. O-fucosylation by Fringe family members within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions. Notch1 receptor undergoes post- translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647aa ligand-binding extracellular region with the 891aa transmembrane, cytoplasmic portion. Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM17) and the presenilin-dependent gamma-secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes.

Product Categories/Family for anti-NOTCH1 antibody

Antibodies; Abs to Notch Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 148833508
• NCBI GeneID: 4851
• NCBI Accession #: NP_060087.3
• NCBI GenBank Nucleotide #: NM_017617.3
• UniProt Accession #: P46531; Q59ED8; Q5SXM3; Q6IAD4; Q9NXX4
• Molecular Weight: 272,505 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 1 preproprotein
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: NOTCH1
• NCBI Official Synonym Symbols: hN1; TAN1
• NCBI Protein Information: neurogenic locus notch homolog protein 1; OTTHUMP00000022594; Notch homolog 1, translocation-associated; translocation-associated notch protein TAN-1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH1
• UniProt Synonym Gene Names: TAN1
• UniProt Entry Name: NOTC1_HUMAN

NCBI Description

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq]

Uniprot Description

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral; Receptor, misc.; Oncoprotein; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; Golgi membrane; endoplasmic reticulum membrane; cell surface; plasma membrane; extracellular region; integral to membrane; acrosome; cytosol; nucleus; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; calcium ion binding; receptor activity; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; heart development; positive regulation of JAK-STAT cascade; response to lipopolysaccharide; response to corticosteroid stimulus; cell differentiation in spinal cord; negative regulation of BMP signaling pathway; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; oligodendrocyte differentiation; negative regulation of osteoblast differentiation; positive regulation of astrocyte differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of keratinocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; organ regeneration; Notch receptor processing; keratinocyte differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; positive regulation of epithelial cell proliferation; foregut morphogenesis; endoderm development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; tissue regeneration; forebrain development; positive regulation of cell proliferation; cardiac muscle morphogensis; heart looping; regulation of somitogenesis; positive regulation of BMP signaling pathway; mesenchymal cell development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; immune response; gene expression; spermatogenesis; sprouting angiogenesis; auditory receptor cell fate commitment; negative regulation of myoblast differentiation; positive regulation of cell migration; lung development

Disease: Adams-oliver Syndrome 5; Aortic Valve Disease 1