Mouse anti-Human NFIC Monoclonal Antibody | anti-NFIC antibody

NFIC Antibody

Cat. Num. AAA9215437

• Gene Names: NFIC; CTF; NFI; CTF5; NF-I
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Synonyms: NFIC; Monoclonal Antibody; NFIC Antibody; Purified Mouse Monoclonal Antibody (Mab); 1110019L22Rik; 1500041O16Rik; AA589446; AI746521; CAAT box transcription factor; CCAAT binding transcription factor; CCAAT box binding transcription factor; CCAAT-box-binding transcription factor; CNFI C; CTF; CTF5; MGC137374; MGC20153; NF I; NF I/C; NF-I/C; NF1 C; NF1-C; NF1C; NFI; NFI-C; NFI/C; NFIC_HUMAN; Nuclear factor 1; Nuclear factor 1 C type; Nuclear factor 1 C-type; Nuclear factor 1/C; Nuclear factor I/C; TGGCA binding protein; TGGCA-binding protein; Transcription factor NFIC.; anti-NFIC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: ascites
• Sequence Length: 508

• Applicable Applications for anti-NFIC antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB ~~ 1:200; ICC ~~ 1:200
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).)

Related Product Information for anti-NFIC antibody

Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

References

Santoro C.,et al.Nature 334:218-224(1988). Wenzelides S.,et al.Nucleic Acids Res. 24:2416-2421(1996). Ota T.,et al.Nat. Genet. 36:40-45(2004). Grimwood J.,et al.Nature 428:529-535(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 350529396
• NCBI GeneID: 4782
• NCBI Accession #: NP_001231931.1
• NCBI GenBank Nucleotide #: NM_001245002.1
• UniProt Accession #: P08651; P08652; Q14932; Q9UPJ3; Q9UPJ9; Q9UPK0; Q9UPK1; A8K1H0; B7Z4U5; B7Z9C3; K7EMU1
• Molecular Weight: 55675
• NCBI Official Full Name: nuclear factor 1 C-type isoform 1
• NCBI Official Synonym Full Names: nuclear factor I/C (CCAAT-binding transcription factor)
• NCBI Official Symbol: NFIC
• NCBI Official Synonym Symbols: CTF; NFI; CTF5; NF-I
• NCBI Protein Information: nuclear factor 1 C-type
• UniProt Protein Name: Nuclear factor 1 C-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIC
• UniProt Synonym Gene Names: NFI; NF1-C; Nuclear factor 1/C; CTF; NF-I/C; NFI-C
• UniProt Entry Name: NFIC_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NFI-C: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: double-stranded DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; odontogenesis of dentine-containing teeth